Use of the surface proteins GapC and Mig of Streptococcus dysgalactiae as potential protective antigens against bovine mastitis
Use of the surface proteins GapC and Mig of Streptococcus dysgalactiae as potential protective antigens against bovine mastitis
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DOI：
10.1139/w04-016
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发表时间：
2004-06-01
影响因子：
2.8
通讯作者：
Perez-Casal, J
中科院分区：
文献类型：
--
作者：
Bolton, A;Song, XM;Perez-Casal, J
停乳链球菌是泌乳和非泌乳奶牛乳腺炎的重要病原体，引起乳腺严重炎症反应，给乳制品行业造成重大经济损失。在干奶牛模型中测试了来自停乳链球菌菌株 SDG8 的两种蛋白质针对同源细菌攻击的保护能力。第一个是牛纤溶酶受体蛋白 (GapC)，它与 A 组链球菌的纤溶酶结合 Plr 蛋白具有 99.4% 的序列同一性。第二种蛋白质产物是 Mig，一种存在于 SDG8 细胞表面的 α(2)(-)M、IgG 和 IgA 结合蛋白。我们通过测量体细胞数量并评估攻击后乳腺分泌物中攻击菌株的存在，研究了纯化重组形式的 GapC 和 Mig 的免疫效果。在该模型中，我们发现，虽然 GapC 免疫动物中含有 SDG8 的细胞数量显着减少，但 Mig 免疫动物中没有，但 GapC 和 Mig 免疫动物中乳头分泌物的体细胞计数均显着减少。
Streptococcus dysgalactiae is a significant pathogen associated with bovine mastitis in lactating and nonlactating dairy cows, causing a severe inflammatory response of the mammary gland, which results in major economic losses to the dairy industry. Two proteins from S. dysgalactiae strain SDG8 were tested for their protective capacity against a homologous bacterial challenge in a dry cow model. The first was a bovine plasmin receptor protein (GapC), which shares 99.4% sequence identity to the plasmin-binding Plr protein of group A streptococci. The second protein product was Mig, a alpha(2)(-)M-, IgG-, and IgA-binding protein present on the cell surface of SDG8. We investigated the efficacy of immunization with purified recombinant forms of GapC and Mig by measuring the number of somatic cells and assessing the presence of the challenge strain in mammary secretions following challenge. In this model, we found that, although the number of quarters containing SDG8 was significantly reduced in the GapC- but not in the Mig-immunized animals, the somatic cell counts from teat secretions were significantly decreased in both the GapC and Mig vaccinates.