Downregulation of Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 in Oral Squamous Cell Carcinoma: Correlation with Tumor Progression and Poor Prognosis
Downregulation of Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 in Oral Squamous Cell Carcinoma: Correlation with Tumor Progression and Poor Prognosis
复制标题
口腔鳞状细胞癌中癌胚抗原相关细胞粘附分子 1 的下调：与肿瘤进展和不良预后的相关性
作者：
K. Shinozuka;K. Uzawa;K. Fushimi;Yukio Yamano;M. Shiiba;H. Bukawa;H. Yokoe;H. Tanzawa
目的：比较口腔鳞状细胞癌细胞系和正常口腔角质形成细胞的基因表达谱，寻找口腔鳞状细胞癌治疗靶点的相关基因。方法：应用Affymetrix基因芯片分析口腔鳞状细胞癌的基因表达谱。识别出的基因被一种独创性的路径分析工具分析，以识别相互作用的基因网络。进一步研究了该候选基因在口腔鳞癌来源的细胞系和原代口腔鳞癌中的表达情况。结果：芯片数据鉴定了188个口腔鳞癌细胞系中表达下调的基因，并产生了与表达变化相关的遗传途径。在定位到该网络中具有最高意义的基因中，进一步分析了癌胚抗原相关细胞黏附分子1(CEACAM1)。口腔鳞癌来源的细胞系中CEACAM1mRNA和蛋白的表达与正常口腔角质形成细胞相比明显下调。免疫组织化学分析显示CEACAM1中原代口腔鳞状细胞明显减少。CEACAM1的表达与TNM分期有关。我们还发现CEACAM1阴性表达对无病(p=0.036)和总存活率(p=0.032)均有显著意义。结论：CEACAM1的表达抑制可能促进口腔鳞癌的进展，并可能预示着口腔鳞癌患者预后不良。
Objective: To identify genes associated with therapeutic targets of oral squamous cell carcinoma (OSCC), we compared gene expression profiles in OSCC-derived cell lines with human normal oral keratinocytes. Methods: We analyzed the gene expression profiles of OSCCs using Affymetrix GeneChip analysis. The identified genes were analyzed by an Ingenuity Pathway Analysis tool to identify networks of interacting genes. A candidate gene was further evaluated for the expression status of the mRNA and protein in OSCC-derived cell lines and primary OSCCs. Results: The microarray data identified 188 genes downregulated in OSCC-derived cell lines, and the genetic pathways associated with expression changes were generated. Among the genes mapped to the network with the highest significance, carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) was analyzed further. CEACAM1 mRNA and protein were frequently downregulated in OSCC-derived cell lines compared with human normal oral keratinocytes. Immunohistochemical analysis showed that primary OSCCs were significantly decreased in CEACAM1. Moreover, CEACAM1 expression was correlated with the TNM staging. We also found that CEACAM1-negative expression was significant both for disease-free (p = 0.036) and overall survival (p = 0.032). Conclusion: Repression of CEACAM1 could contribute to cancer progression and may indicate a poor prognosis for patients with OSCC.
人前列腺发育过程中上皮细胞粘附分子（C-CAM）的一致表达和前列腺癌中表达的丧失：作为肿瘤抑制因子的含义。
影响因子：
11.2
作者：
Kleinerman,DI;Troncoso,P;Lin,SH;Pisters,LL;Sherwood,ER;Brooks,T;vonEschenbach,AC;Hsieh,JT
通讯作者：
Hsieh,JT
结直肠癌进展过程中 KAI1 表达缺失。
影响因子：
11.2
作者：
Lombardi,DP;Geradts,J;Foley,JF;Chiao,C;Lamb,PW;Barrett,JC
通讯作者：
Barrett,JC