Effects of immobilization and dynamic compression on intervertebral disc cell gene expression in vivo
Effects of immobilization and dynamic compression on intervertebral disc cell gene expression in vivo
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DOI：
10.1097/00007632-200305150-00004
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发表时间：
2003-05-15
期刊：
影响因子：
3
通讯作者：
Iatridis, JC
中科院分区：
文献类型：
--
作者：
MacLean, JJ;Lee, CR;Iatridis, JC
研究设计.使用鼠尾模型进行椎间盘细胞对动态压缩和固定的反应的体内分析。评估制动和短期动态压迫对椎间盘细胞合成代谢和分解代谢基因表达的影响。体内施加的静态压缩载荷以剂量依赖性方式改变椎间盘基质的组成和细胞活力。体内动态压缩的影响，这是一个更生理负荷，和报告的风险因素腰痛尚未调查。将lilizarov型装置植入大鼠尾部，并用于确定72小时固定（n = 6）、2小时动态压缩（1 MPa/0.2Hz）（n = 8）和固定后压缩的耦合效应（n = 8）的效果。实时逆转录-聚合酶链反应被用来测量相对于内部对照组和假手术组（n = 7）的合成代谢和分解代谢基因水平的变化。固定和动态压缩影响合成代谢和分解代谢基因，1型和2型胶原蛋白的整体下调和纤维环中聚集蛋白聚糖酶、胶原酶和基质溶解素的上调。固定和压缩的影响似乎是1型和2型胶原纤维环的加法，但不是在核，而不是分解代谢基因。短期动态压迫和制动改变大鼠椎间盘基因表达。在研究椎间盘对负荷的反应时，有必要研究合成代谢和分解代谢途径，并考虑应变史。
Study Design. An in vivo analysis of the intervertebral disc's cellular response to dynamic compression and immobilization was performed using a rat-tail model.Objective. To assess the effects of immobilization and short-term dynamic compression on intervertebral disc cell expression of anabolic and catabolic genes.Summary of Background Data. Static compressive loads applied in vivo alter the composition of the disc matrix and cell viability in a dose-dependent manner. The effects of in vivo dynamic compression, which is a more physiologic load, and reported risk factor for low back pain have not been investigated.Methods. An llizarov-type device was implanted on the rat tail and used to determine the effects from 72 hours of immobilization (n = 6), 2 hours of dynamic compression (1 MPa/0.2 Hz) (n = 8), and the coupled effect of immobilization followed by compression (n = 8). Real-time reverse transcription-polymerase chain reaction was used to measure changes in anabolic and catabolic gene levels relative to both internal control subjects and a sham-operated group (n = 7).Results. Immobilization and dynamic compression affect anabolic and catabolic genes, with an overall down-regulation of types 1 and 2 collagen and upregulation of aggrecanase, collagenase, and stromelysin in the anulus. The effects of immobilization and compression appear to be additive for collagen types 1 and 2 in the anulus, but not in the nucleus, and not for catabolic genes.Conclusions. Short-duration dynamic compression and immobilization alter gene expression in the rat disc. In studying the response of the disc to loading, it is necessary to look at both anabolic and catabolic pathways, and to consider strain history.