Light microscopic evidence of hair cell regeneration after gentamicin toxicity in chick cochlea.
Light microscopic evidence of hair cell regeneration after gentamicin toxicity in chick cochlea.
复制标题
DOI：
10.1001/archotol.1987.01860100036017
复制
发表时间：
1987-10
期刊：
影响因子：
--
通讯作者：
R. Cruz;P. Lambert;E. Rubel
中科院分区：
文献类型：
--
作者：
R. Cruz;P. Lambert;E. Rubel
本研究考察了在给药10天后雏鸡基底乳头毛细胞丢失的时间模式。分别在雏鸡11、18、25、32日龄时处死。基底乳头包埋于塑料中，连续切片进行光镜分析。在整个乳头长度上每隔100微米进行一次毛细胞计数。在11日龄的雏鸡中记录了显著的毛细胞损失，并随着时间的推移向顶部扩散，在18日龄的雏鸡中达到最大的损失。与对照组相比，这些雏鸡的毛细胞减少了36%。有趣的是，在25天和32天大的动物中，毛细胞数量似乎逐渐部分恢复正常。
This study examines the temporal pattern of hair cell loss in the chick basilar papilla following ten days of gentamicin administration in hatchling chicks. Chicks were subsequently killed at ages 11, 18, 25, and 32 days. The basilar papillae were embedded in plastic and serially sectioned for light microscopic analysis. Hair cell counts were obtained at 100-microM intervals throughout the length of the papilla. Significant hair cell loss was documented basally in the 11-day-old chicks, and spread apically over time to maximal loss in the 18-day-old animals. Relative to the control chicks, there was a 36% hair cell loss in these animals. Interestingly, there appears to be a progressive partial recovery of the normal hair cell counts in the 25- and 32-day-old animals.