MACROPHAGE FUNCTION IN VITAMIN C-DEFICIENT GUINEA-PIGS
MACROPHAGE FUNCTION IN VITAMIN C-DEFICIENT GUINEA-PIGS
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DOI：
10.1093/ajcn/29.7.762
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发表时间：
1976-01-01
影响因子：
7.1
通讯作者：
WALDMAN, RH
中科院分区：
文献类型：
--
作者：
GANGULY, R;DURIEUX, MF;WALDMAN, RH
给豚鼠喂食维生素C缺乏的饮食，然后在不同的时间对它们的腹膜细胞进行生物活性测试。缺乏维生素C的动物的血清水平表明，随着时间的推移，抗坏血酸缺乏症的进展状态，这与坏血病的临床体征和症状。从缺陷动物的腹腔中获得的巨噬细胞较少，并且发现的巨噬细胞尺寸较小。它们对细菌细胞的吞噬作用无明显损害。然而，与正常细胞相比，巨噬细胞在玻璃表面上表现出显著减少的迁移。在体外添加维生素C部分逆转这种减少迁移。
Guinea pigs were fed a vitamin C-deficient diet and at various times thereafter their peritoneal cells were tested for biological activity. The serum levels of vitamin C in the deficient animals indicated a progressive state of ascorbic acid deficiency with time and this correlated well with clinical signs and symptoms of scurvy. Fewer macrophages were obtained from the peritoneal cavities of deficient animals and those found were smaller in size. They showed no significant impairment in phagocytosis of bacterial cells. The macrophages, however, exhibited significantly reduced migration on glass surface as compared to the normal cells. In vitro addition of vitamin C partially reversed this reduced migration.