Fluorescence lifetime measurement via a radionuclide-scintillation light source and analog cross correlation.
Fluorescence lifetime measurement via a radionuclide-scintillation light source and analog cross correlation.
复制标题
通过放射性核素闪烁光源和模拟互相关进行荧光寿命测量。
DOI：
10.1021/ac961130m
复制
发表时间：
1997
影响因子：
7.4
通讯作者：
Hieftje,GM
中科院分区：
文献类型：
--
作者：
Burden,DL;Hobbs,SE;Hieftje,GM
β-Emitting90Sr与塑料闪烁体一起用于产生用于荧光寿命分析的激发光脉冲。这种光源比传统上采用的激发源（例如激光器或脉冲闪光灯）更便宜、更紧凑并且可靠得多。来自该光源的脉冲串在幅度和时间上随机变化。互相关信号分析对于这样的源是理想的，因为与其他时域技术不同，互相关利用了其随机性的全部优点。在这里，我们报告的仪器和方法的建设，使荧光寿命测量通过新的源和模拟相关处理器。尽管基于闪烁器的激发源的光强度相对较低，但可以产生足够的信号水平。三个荧光团的荧光寿命测量与1毫居里的放射性核素，以证明寿命范围从小于1.5到28纳秒。长寿命测量需要额外的校准步骤，以补偿延迟电缆的能量损失。发现当前仪器的光收集效率不理想地低;建议在仪器光学器件中进行改进，这将提高收集效率并增强检测能力。
β-Emitting90Sr is used with a plastic scintillator to produce excitation-light pulses for fluorescence lifetime analysis. This light source is less expensive, more compact, and much more reliable than traditionally employed excitation sources such as lasers or pulsed flash lamps. The pulse train from this light source varies randomly in amplitude and time. Cross-correlation signal analysis is ideal for such a source because, unlike other time domain techniques, cross correlation takes complete advantage of its random nature. Here we report on the construction of an instrument and the methods employed to make fluorescence lifetime measurements via the new source and an analog correlation processor. Although the light intensity of the scintillator-based excitation source is comparatively low, an adequate signal level can be generated. The fluorescence lifetimes of three fluorophores are measured with a 1-mCi radionuclide to demonstrate a lifetime range from less than 1.5 to 28 ns. Long-lifetime measurements require an extra calibration step in order to compensate for delay cable energy loss. The light collection efficiency of the current instrument was found to be undesirably low; improvements in the instrument optics are suggested that will increase the collection efficiency and enhance the detection capability.