In vitro Assays for Measuring Endothelial Permeability by Transwells and Electrical Impedance Systems
In vitro Assays for Measuring Endothelial Permeability by Transwells and Electrical Impedance Systems
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DOI：
10.21769/bioprotoc.2273
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发表时间：
2017-05-05
期刊：
影响因子：
0.8
通讯作者：
Yeh, Trai-Ming
中科院分区：
文献类型：
--
作者：
Chen, Hong-Ru;Yeh, Trai-Ming
血管渗漏是感染性休克、病毒性出血热、肿瘤转移和缺血再灌注损伤等疾病的重要特征。因此，建立用于测量内皮通透性的测定将提供对此类疾病的建立或进展的了解。在此，我们提供了transwell渗透性测定法和电阻抗传感法来研究内皮细胞的渗透性。通过这些方法，可以确定分子对内皮通透性的影响。
Vascular leakage is an important feature in several diseases, such as septic shock, viral hemorrhagic fever, cancer metastasis and ischemia-reperfusion injuries. Thus establishing assays for measuring endothelial permeability will provide insight into the establishment or progression of such diseases. Here, we provide transwell permeability assay and electrical impedance sensing assay for studying endothelial permeability in vitro. With these methods, the effect of a molecule on endothelial permeability could be defined.