Radical free crosslinking of direct-write 3D printed hydrogels through a base catalyzed thiol-Michael reaction
Radical free crosslinking of direct-write 3D printed hydrogels through a base catalyzed thiol-Michael reaction
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DOI：
10.1039/c9py00953a
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发表时间：
2019-11-28
影响因子：
4.6
通讯作者：
Smaldone, Ronald A.
中科院分区：
文献类型：
--
作者：
Berry, Danielle R.;Diaz, Brisa K.;Smaldone, Ronald A.
在这项工作中，我们描述了一种制造3D打印水凝胶的方法，这种水凝胶是机械稳定的，而不使用潜在的细胞毒性自由基化学。为了实现这一目标，我们利用碱性（pH 8.2）磷酸盐缓冲盐水（PBS）在二丙烯酸Pluronic F127和多功能硫醇交联剂之间催化的硫醇-迈克尔反应。我们发现，由于缓冲液的离子强度使Pluronic胶束稳定，打印分辨率可以保持不变。这些水凝胶表现出高拉伸性（类似于750%）以及可调的机械性能。我们证明了基于胶束的独立3D物体可以通过非自由基途径通过增加离子强度稳定溶液中的胶束来制造。
In this work we describe a method of fabricating 3D printed hydrogels which are mechanically stabilized without the use of potentially cytotoxic radical chemistry. To achieve this, we utilized a thiol-Michael reaction catalyzed by basic (pH 8.2) phosphate buffered saline (PBS) between diacrylated Pluronic F127 and multifunctional thiol crosslinkers. We showed that the print resolution could be conserved due to stabilization of Pluronic micelles as a result of the ionic strength of the buffer. These hydrogels exhibited high stretchability (similar to 750%) as well as tunable mechanical properties. We demonstrated that micelle-based free-standing 3D objects can be fabricated through non-radical pathways by stabilizing the micelles in solutions with increased ionic strength.