Analysis of porous carbon biocathodes via three-dimensional impedance spectroscopy using a double channel transmission line model.
Analysis of porous carbon biocathodes via three-dimensional impedance spectroscopy using a double channel transmission line model.
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使用双通道传输线模型通过三维阻抗谱分析多孔碳生物阴极。
DOI：
10.1016/j.bios.2021.113014
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发表时间：
2021
影响因子：
12.6
通讯作者：
M. Itagaki
中科院分区：
文献类型：
--
作者：
Isao Shitanda;H. Inoue;Yukihiro Yoshihata;N. Loew;M. Itagaki
近年来，多孔碳电极大大提高了生物燃料电池和生物传感器的性能。本文提出了一种新的多孔酶电极的原位分析方法。通过结合三维（3D）阻抗测量和双通道传输线模型，可以评估多孔酶电极在操作过程中的稳定性。所提出的方法可以区分酶和介体反应的功能稳定性和电极的一般结构稳定性。我们证明了这种方法，通过评估胆红素氧化酶改性的碳布（CC）电极与氧化镁（MgO）模板的碳涂层。在CC电极的情况下，在前500秒内电荷转移电阻的显著增加表明酶和介体的洗脱。当CC在酶改性之前用MgO模板化的碳涂覆时，电荷转移电阻保持恒定，表明酶和介体的洗脱的有效抑制。两种电极的双电层电容值表明，它们的一般电极结构是稳定的分析过程中。因此，所提出的分析方法，基于3D阻抗，可以是一个强大的工具，用于同时检测酶电极的一般电极结构和电极表面上的活性酶和介质的量的可能变化。
Porous carbon electrodes have considerably improved the performance of biofuel cells and biosensors in recent years. In this paper, we propose a novelin-situanalysis method for porous enzyme electrodes. By combining three-dimensional (3D) impedance measurement and a double-channel transmission line model, the stability of porous enzyme electrodes during operation can be evaluated. The proposed method can distinguish between the functional stability of the enzyme and mediator reaction and the general structural stability of the electrode. We demonstrated this method by evaluating bilirubin oxidase-modified carbon cloth (CC) electrodes with and without a magnesium oxide (MgO)-templated carbon coating. In case of the CC electrode, a remarkable increase in the charge transfer resistance within the first 500 s indicated the elution of the enzyme and mediator. When the CC was coated with MgO-templated carbon before enzyme modification, the charge transfer resistance remained constant, indicating an effective suppression of the elution of the enzyme and mediator. The electric double-layer capacitance values of both electrodes indicated that their general electrode structures were stable during the analysis. Thus, the proposed analytical method, based on 3D impedance, can be a powerful tool for simultaneously detecting possible changes in the general electrode structure of enzyme electrodes and in the amount of active enzymes and mediators on the electrode surface.
基于MgO模板碳的酶生物燃料电池
DOI：
--
发表时间：
2016
期刊：
影响因子：
--
作者：
Ayumu Niiyama;Seiya Tsujimura;辻村清也;Seiya Tsujimura;Seiya Tsujimura
通讯作者：
Seiya Tsujimura
基于MgO模板多孔碳的葡萄糖/O2酶生物燃料电池
DOI：
--
发表时间：
2016
期刊：
影响因子：
--
作者：
Seiya Tsujimura; Ayumu Niiyama
通讯作者：
Ayumu Niiyama