Protein carbonylation and heat shock response in Ruditapes decussatus following p,p′-dichlorodiphenyldichloroethylene (DDE) exposure:: A proteomic approach reveals that DDE causes oxidative stress
Protein carbonylation and heat shock response in Ruditapes decussatus following p,p′-dichlorodiphenyldichloroethylene (DDE) exposure:: A proteomic approach reveals that DDE causes oxidative stress
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DOI：
10.1016/j.aquatox.2005.10.011
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发表时间：
2006-04-20
影响因子：
4.5
通讯作者：
Sheehan, D
中科院分区：
文献类型：
--
作者：
Dowling, V;Hoarau, PC;Sheehan, D
研究了对二氯二苯基二氯乙烯(DDE)对十字贝鳃、外套膜和消化腺的蛋白质羰化作用和热休克蛋白(HSP；60、70和90 kDa)水平的影响。热休克反应通过使用热休克蛋白(HSPs)特异性抗体的免疫印迹来测量。对单个条带的密度分析表明，除了DDE处理的外套膜中出现HSP90外，对照和处理样本之间没有差异。用2，4-二硝基苯肼衍生化、双向凝胶电泳法和Western blotting法测定蛋白质的羧化含量。用二硝基苯酚特异性抗体免疫印迹显示，外套膜和消化腺对DDE的反应在程度和数量上都有很大的差异，而鳃则没有受到影响。这些结果首次证明了DDE在文蛤中引起组织特异性的活性氧物种的形成。(C)2005 Elsevier B.V.保留所有权利。
Protein carbonylation and levels of heat shock proteins (hsp; 60, 70 and 90 kDa) were measured in gill, mantle and digestive gland of Ruditapes decussatus following exposure to p,p'-dichlorodiphenyldichloroethylene (DDE). Heat shock response was measured by immunoblotting using antibodies specific to heat shock proteins (hsps). Densitometry analysis of individual bands revealed no difference between control and treated samples except appearance of hsp90 in DDE-treated mantle. Carbonylated protein content was determined following 2,4-dinitrophenylhydrazine derivatization and two-dimensional electrophoresis coupled with western blotting. Immunoblotting with dinitrophenol-specific antibody revealed extensive differences in both extent and number of carbonylated proteins in mantle and digestive gland in response to DDE while gill was unaffected. These results demonstrate for the first time that DDE causes tissue-specific formation of reactive oxygen species in clams. (c) 2005 Elsevier B.V. All rights reserved.