Expression of interleukin-7 and its receptor in thyroid lymphoma
Expression of interleukin-7 and its receptor in thyroid lymphoma
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DOI：
10.1038/labinvest.3780157
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发表时间：
2000-10-01
影响因子：
5
通讯作者：
Aozasa, K
中科院分区：
文献类型：
--
作者：
Takakuwa, T;Nomura, S;Aozasa, K
病理流行病学研究表明，甲状腺淋巴瘤（TL）发生于受慢性淋巴细胞性甲状腺炎（CLTH）影响的甲状腺。CLTH中产生的细胞因子可能在淋巴瘤发生中起关键作用，因为先前的报道表明细胞因子在淋巴瘤发生中起重要作用。我们通过RT-PCR检测了IL-7（一种主要作用于血淋巴系统细胞的多效性细胞因子）和IL-7受体（IL-7 R）在TL和CLTH中的表达。IL-7特异性转录物在TL中比在CLTH病变中更频繁地检测到（p < 0.01）。IL-7在TL中的表达高于CLTH。IL-7 R和共同的γ链表达在所有TL，但在所有CLTH病变，在相似的水平。建立了IL-7的原位杂交检测方法。IL-7原位杂交显示TL和CLTH病变中淋巴滤泡和滤泡间区的生发中心和外套层细胞间的细胞质信号。在TL的淋巴瘤区，发现散在的表达IL-7的大小淋巴样细胞的数量相似，TL的10个视野中IL-7表达细胞的数量（119.4 ± 10.6个细胞）显著高于CLTH病变（43.1 ± 4.6个细胞）（p < 0.001）。这些发现表明IL-7在TL的发展中的致病作用。原位杂交结果显示，生发中心细胞、滤泡间细胞和淋巴瘤细胞表达IL-7 R，而套层细胞不表达。由于CLTH形成的淋巴滤泡和滤泡间区的淋巴细胞表达IL-7，TL细胞可能通过其自身的IL-7和IL-7 R以及通过反应性淋巴细胞的IL-7增殖。
Patho-epidemiological studies have shown that thyroid lymphomas (TL) develop in thyroids affected by chronic lymphocytic thyroiditis (CLTH). Cytokines produced in CLTH might play a pivotal role for lymphomagenesis, because previous reports indicate an important role of cytokines in lymphomagenesis. We examined the expression of interleukin-7 (IL-7), a pleiotropic cytokine that acts mainly on cells of the hematolymphoid system, and IL-7 receptor (IL-7R) in both TL and CLTH by RT-PCR. IL-7-specific transcripts were detected more frequently in TL than in CLTH lesions (p < 0.01). IL-7 expression was higher in TL than in CLTH. IL-7R and the common gamma chain were expressed in all but one TL and in all CLTH lesions, in similar levels. We established the sensitive in situ hybridization (ISH) method for detection of IL-7. ISH for IL-7 revealed cytoplasmic signals among cells in the germinal center and mantle zone of lymphoid follicles and interfollicular areas in the TL and CLTH lesions. In the lymphomatous areas of TL, similar numbers of scattered large and small lymphoid cells expressing IL-7 were found. The number of IL-7-expressing cells counted in 10 fields in TL (119.4 10.6 cells) was significantly higher than found in CLTH lesions (43.1 +/- 4.6) (p < 0.001). These findings suggest a pathogenetic role for IL-7 in the development of TL. ISH showed that the germinal center cells, interfollicular cells, and lymphoma cells expressed IL-7R, but mantle zone cells did not. Because lymphoid cells in lymphoid follicles and the interfollicular area formed in CLTH expressed IL-7, TL cells might proliferate via their own IL-7 and IL-7R, and via IL-7 from reactive lymphoid cells.