Profiling of adhesive properties of the agglutinin-like sequence (ALS) protein family, a virulent attribute of Candida albicans
Profiling of adhesive properties of the agglutinin-like sequence (ALS) protein family, a virulent attribute of Candida albicans
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DOI：
10.1111/j.1574-695x.2012.00941.x
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发表时间：
2012-06-01
影响因子：
--
通讯作者：
Ueda, Mitsuyoshi
中科院分区：
文献类型：
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作者：
Aoki, Wataru;Kitahara, Nao;Ueda, Mitsuyoshi
白色念珠菌通常存在于几乎所有人类中，但可在免疫功能低下的患者中引起致命疾病。与白色念珠菌相似的凝集素样序列（ALS）基因家族被认为对医疗器械上生物膜的形成很重要。在这里，我们克隆了所有ALS基因，并确定其基因产物的结合特性的细胞表面工程的酿酒酵母。大多数ALS同系物具有结合聚丙烯、硼硅酸盐玻璃和聚氯乙烯的能力，这些材料通常用作医疗器械中的材料。然而，ALS同系物不能结合聚氨酯、聚甲基丙烯酸甲酯、聚四氟乙烯或钛。这些发现将有助于开发生物膜抗性医疗器械。
Candida albicans is normally present in nearly all humans but can cause fatal diseases in immunocompromised patients. The agglutinin-like sequence (ALS) gene family of C.similar to albicans has been suggested to be important for biofilm formation on medical devices. Here, we cloned all ALS genes and determined the binding properties of their gene products by cell surface engineering of Saccharomyces cerevisiae. Most of the ALS homologues had the ability to bind polypropylene, borosilicate glass and polyvinyl chloride, which are often used as materials in medical devices. However, ALS homologues were not able to bind polyurethane, polymethyl methacrylate, polytetrafluoroethylene or titanium. These findings will aid in the development of biofilm-resistant medical devices.