High-resolution HLA typing for the DQB1 gene by sequence-based typing.
High-resolution HLA typing for the DQB1 gene by sequence-based typing.
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通过基于序列的分型对 DQB1 基因进行高分辨率 HLA 分型。
DOI：
10.1111/j.1399-0039.1997.tb02872.x
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
E. M. Berg‐Loonen
中科院分区：
文献类型：
--
作者：
C. Voorter;M. Kik;E. M. Berg‐Loonen
多态性基因的理想高分辨率分型策略是基于序列的分型。已经针对HLA II类基因DRB 1、DRB 3/4/5和DPB 1开发了基因组DNA的SBT。对于DQB 1基因，基于序列的分型方法被证明会引起许多问题。针对这些问题，设计了不同的引物对DQB 1基因外显子2进行扩增和测序。使用几种引物组合，在DQB 1 *02/*03和DQB 1 *02/*04杂合子个体中观察到优先扩增。在许多情况下偏好DQB 1 *02，但也可以证明DQB 1 *03或 *04，偶尔导致等位基因脱落。选择了最佳引物组合，并成功地用于对DQB 1 *02、*03和 *04杂合子个体进行分型。为了区分DQB 1 *0201和 *0202，开发了用于扩增和测序外显子3的引物，并获得了正确的亚型。通过等位基因特异性扩增和测序解决了DQB 1 *0301/*0302和DQB 1 *0303/*0304的模糊分型。总共有258个人被完全分型为他们的DQB 1亚型。所有样本先前已通过PCR-SSP和血清学分型。所有受试个体均获得一致的分型结果。检测到的DQB 1等位基因包括 *0501、*0502、*0503、*0601、*0602、*0603、*0604、*0609、*0201、*0202、*0301、*0302、*0303、*0304、*0401和 *0402。基于序列的分型的DQB 1基因被证明是一个可靠的分型策略分配不同的DQB 1等位基因后，密集的选择引物和测试条件。
The ideal high-resolution typing strategy for polymorphic genes is sequence-based typing. SBT of genomic DNA has been developed for the HLA class II genes DRB1, DRB3/4/5 and DPB1. For the DQB1 gene the sequence-based typing method was shown to cause a number of problems. To resolve those problems, different primers to amplify and sequence exon 2 of DQB1 were designed and tested. With several primer combinations, preferential amplification was observed in individuals heterozygous for DQB1*02/*03 and DQB1*02/*04. The preference was for DQB1*02 in many instances but could also be demonstrated for DQB1*03 or *04 and resulted occasionally in allelic drop-out. The best primer combination was selected and successfully used to type individuals heterozygous for DQB1*02, *03 and *04. To distinguish DQB1*0201 and *0202, primers for amplification and sequencing of exon 3 were developed and correct subtyping was obtained. The ambiguous typing DQB1*0301/*0302 and DQB1*0303/*0304 was resolved by allele-specific amplification and sequencing. A total of 258 individuals were fully typed for their DQB1 subtypes. All samples had been previously typed by PCR-SSP and serology. Concordant typing results were obtained for all individuals tested. The DQB1 alleles detected included *0501, *0502, *0503, *0601, *0602, *0603, *0604, *0609, *0201, *0202, *0301, *0302, *0303, *0304, *0401 and *0402. Sequence-based typing of the DQB1 gene proved a reliable typing strategy for assignment of the different DQB1 alleles after intensive selection of primers and test conditions.
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人类主要组织相容性复合体的 DC 和 DX 亚区的基因组织。
DOI：
10.1073/pnas.82.10.3410
发表时间：
1985
影响因子：
11.1
作者：
Okada,K;Boss,JM;Prentice,H;Spies,T;Mengler,R;Auffray,C;Lillie,J;Grossberger,D;Strominger,JL
通讯作者：
Strominger,JL
通过寡分型检测 14 个 HLA-DQB1 等位基因。
影响因子：
2.7
作者：
Molkentin,J;Gorski,J;Baxter-Lowe,LA
通讯作者：
Baxter-Lowe,LA
影响因子：
2.7
作者：
SANTAMARIA, P;BOYCEJACINO, MT;RICH, SS
通讯作者：
RICH, SS
罕见 DR2/LD-5a/DQw3 HLA II 类单倍型的分子研究。
DOI：
--
发表时间：
1988
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Liu,CP;Bach,FH;Wu,SK
通讯作者：
Wu,SK