AN EVOLUTIONARILY CONSERVED PROTEIN-BINDING SEQUENCE UPSTREAM OF A PLANT LIGHT-REGULATED GENE
AN EVOLUTIONARILY CONSERVED PROTEIN-BINDING SEQUENCE UPSTREAM OF A PLANT LIGHT-REGULATED GENE
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DOI：
10.1073/pnas.85.19.7089
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发表时间：
1988-10-01
影响因子：
11.1
通讯作者：
CASHMORE, AR
中科院分区：
文献类型：
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作者：
GIULIANO, G;PICHERSKY, E;CASHMORE, AR
从番茄（茄科）和拟南芥（芸苔科）幼苗的核提取物中发现了一种蛋白质因子，可以特异性结合植物光调节基因家族的上游序列，该基因编码核酮糖1,5-二磷酸羧化酶/加氧酶（RBCS）的小亚基。来自番茄、豌豆（Pisum sativum, Leguminosae）和拟南芥的RBCS上游序列被该因子识别。因子识别通过短保守序列（G盒）进行，其一致序列为5“- tcttacacgtggcay -3（其中Y为嘧啶）。这个序列不同于之前在红细胞启动子中描述的GT基序。另外两个保守序列，显示出较小程度的进化保守性，在G盒上游发现，但不与G盒结合因子（GBF）结合。G盒内的12个核苷酸足以形成稳定的DNA-GBF复合物。GBF存在于光生长和暗生长的番茄叶片提取物中，但其在根提取物中的含量大大减少。
A protein factor, identified in nuclear extracts obtained from tomato (Lycopersicon esculentum, Solanaceae) and Arabidopsis thaliana (Brassicaceae) seedlings, specifically binds upstream sequences from the plant light-regulated gene family encoding the small subunit of ribulose 1,5-bisphosphate carboxylase/oxygenase (RBCS). RBCS upstream sequences from tomato, pea (Pisum sativum, Leguminosae), and Arabidopsis are recognized by the factor. The factor recognition occurs via a short conserved sequence (G box) whose consensus sequence is 5''-TCTTACACGTGGCAYY-3 (where Y is pyrimidine). This sequence is distinct from the GT motif described previously in RBCS promoters. Two other conserved sequences, showing a lesser degree of evolutionary conservation, are found upstream of the G box but do not bind to the G box binding factor (GBF). Twelve nucleotides within the G box are sufficient for the formation of a stable DNA-GBF complex. GBF is found in both light-grown and dark-adapted tomato leaf extracts, but it is present in greatly reduced amounts in root extracts.