Genetic characterization of two fully sequenced multi-drug resistant plasmids pP10164-2 and pP10164-3 from Leclercia adecarboxylata.
Genetic characterization of two fully sequenced multi-drug resistant plasmids pP10164-2 and pP10164-3 from Leclercia adecarboxylata.
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DOI：
10.1038/srep33982
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发表时间：
2016-09-23
影响因子：
4.6
通讯作者：
Xia P
中科院分区：
文献类型：
--
作者：
Sun F;Zhou D;Sun Q;Luo W;Tong Y;Zhang D;Wang Q;Feng W;Chen W;Fan Y;Xia P
我们先前报道了耐药质粒pP 10164-NDM的完整序列，该质粒携带blaNDM（赋予碳青霉烯耐药）和bleMBL（赋予博莱霉素耐药），该质粒从中国临床分离的脱羧勒克菌P10164中回收。本研究的后续工作发现，该菌株中还存在两个多药耐药质粒pP 10164 -2和pP 10164 -3。对pP 10164 -2和pP 10164 -3进行了全序列测定，结果表明它们携带有丰富的耐药基因，编码对至少10类抗生素（β-内酰胺类）的耐药性。大环内酯类、喹诺酮类、氨基糖苷类、四环素类、氨酚类、季铵化合物、磺胺类、甲氧苄啶和利福平）和7种重金属（汞、银、铜、镍、铬酸盐、砷和碲）。所有这些抗生素抗性基因都与诸如转座子、整合子和基于插入序列的转座单位的移动的元件相关，构成总共三个新的MDR区域，两个在pP 10164 -2中，另一个在pP 10164 -3中。三种抗性质粒pP 10164-NDM、pP 10164 -2和pP 10164 -3的共存使L.脱羧菌P10164倾向于变得广泛耐药。
We previously reported the complete sequence of the resistance plasmid pP10164-NDM, harboring blaNDM (conferring carbapenem resistance) and bleMBL (conferring bleomycin resistance), which is recovered from a clinical Leclercia adecarboxylata isolate P10164 from China. This follow-up work disclosed that there were still two multidrug-resistant (MDR) plasmids pP10164-2 and pP10164-3 coexisting in this strain. pP10164-2 and pP10164-3 were completely sequenced and shown to carry a wealth of resistance genes, which encoded the resistance to at least 10 classes of antibiotics (β-lactams. macrolides, quinolones, aminoglycosides, tetracyclines, amphenicols, quaternary ammonium compounds, sulphonamides, trimethoprim, and rifampicin) and 7 kinds of heavy mental (mercury, silver, copper, nickel, chromate, arsenic, and tellurium). All of these antibiotic resistance genes are associated with mobile elements such as transposons, integrons, and insertion sequence-based transposable units, constituting a total of three novel MDR regions, two in pP10164-2 and the other one in pP10164-3. Coexistence of three resistance plasmids pP10164-NDM, pP10164-2 and pP10164-3 makes L. adecarboxylata P10164 tend to become extensively drug-resistant.
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