Inter- and intra-specific variation in destruxin production by insect pathogenic Metarhizium spp., and its significance to pathogenesis
Inter- and intra-specific variation in destruxin production by insect pathogenic Metarhizium spp., and its significance to pathogenesis
复制标题
DOI：
10.1017/s095375629900146x
复制
发表时间：
2000-04-01
影响因子：
--
通讯作者：
Butt, TM
中科院分区：
文献类型：
--
作者：
Amiri-Besheli, B;Khambay, B;Butt, TM
在绿僵菌中检测到了destruxin产生的种间和种内变异，这对于确定针对昆虫的毒力和/或特异性可能很重要。 M. anisopliae var, anisopliae 菌株产生不同量的destruxins A、B和E，但菌株V220不产生任何destruxins。 M. anisopliae 变种。据报道，majus、M. flavoviride 和 M. album 分别对鞘翅目、直翅目和半翅目有特异性，具有不同的 destruxin 谱，其中以 destruxin A 为主。 M. anisopliae var. 的时间进程研究。 anisopliae V245，destruxin E 水平随时间下降，而 destruxin A 水平增加。毒性最强的菌株Ma23和V245产生大量的destruxins，但一些低毒素生产者也具有毒性，这表明destruxins并不是唯一的致病性决定因素。一些弱至中度致病性菌株在注射到大蜡螟幼虫中时具有高致病性，这表明角质层作为真菌感染屏障的重要性。在感染 M. anisopoliae var. 的 Galleria 幼虫中仅检测到微量的 destruxin A 或 A 和 B 的组合。 anisopliae 或 M. anisopliae var, majus。在感染黄绿分枝杆菌的幼虫中未检测到destruxins。 Destruxin 的产生可能会受到昆虫或培养基中营养物质的影响。
Inter- and intra-specific variation in destruxin production was detected in Metarhizium and may be important in determining virulence and/or specificity against insects. Strains of M. anisopliae var, anisopliae produced different amounts of destruxins A, B and E, but strain V220 did not produce any destruxins. M. anisopliae var. majus, M. flavoviride and M. album which are reported to be specific towards Coleoptera, Orthoptera and Hemiptera respectively, had different destruxin profiles with destruxin A predominant. In time course studies on M. anisopliae var. anisopliae V245, destruxin E levels declined with time while destruxin A levels increased. The most virulent strains, Ma23 and V245, produced large quantities of destruxins but some low toxin producers were also virulent, suggesting that destruxins are not the only pathogenicity determinants. Some weakly to moderately pathogenic strains were highly pathogenic when injected into Galleria mellonella larvae, demonstrating the importance of the cuticle as a barrier to fungal infection. Only trace amounts of destruxin A or a combination of A and B could be detected in Galleria larvae infected with M. anisopoliae var. anisopliae or M. anisopliae var, majus. No destruxins were detected in larvae infected with M. flavoviride. Destruxin production may be influenced by the nutrients in insects or culture media.