Cloning and sequencing of the granulin gene from the Trichoplusia ni granulosis virus.
Cloning and sequencing of the granulin gene from the Trichoplusia ni granulosis virus.
复制标题
粉纹夜蛾颗粒病毒颗粒蛋白基因的克隆和测序。
DOI：
10.1016/0042-6822(85)90267-3
复制
发表时间：
1985
期刊：
影响因子：
3.7
通讯作者：
Beaudreau,GS
中科院分区：
文献类型：
--
作者：
Akiyoshi,D;Chakerian,R;Rohrmann,GF;Nesson,MH;Beaudreau,GS
以克隆的多角体蛋白基因为探针，在黑纹夜蛾病毒基因组DNA中定位了含有颗粒蛋白基因的限制性内切酶片段。对该片段进行了克隆、定位、亚克隆，并测定了编码区及5′和3′侧翼区的序列。虽然颗粒蛋白的大小与核型多角体病毒多角体蛋白非常相似，但颗粒蛋白的N-末端区域表现出高度的变异性，前60个氨基酸与苜蓿银纹夜蛾核型多角体病毒（AcMNPV）多角体蛋白序列的同源性仅为28%。在氨基酸60和氨基酸248的羧基末端之间，序列与多角体蛋白序列非常相似（64%）。总的来说，核苷酸和氨基酸序列分别为58%和53%，相关的AcMNPV。在该基因中没有明显的内含子。
A restriction fragment containing the granulin gene from theTrichoplusia nigranulosis virus was located in a blot of viral genomic DNA using a cloned polyhedrin gene as a probe. This fragment was cloned, mapped, subcloned, and the sequence of the coding region and 5′ and 3′ flanking regions was determined. Although granulin is very similar in size to nuclear polyhedrosis virus polyhedrins, the N-terminal region of granulin demonstrated a high degree of variability with the first 60 amino acids only 28% homologous to theAutographa californicanuclear polyhedrosis virus (AcMNPV) polyhedrin sequence. Between amino acid 60 and the carboxyterminus at amino acid 248, the sequence was very similar (64%) to polyhedrin sequences. Overall the nucleotide and amino acid sequences were 58 and 53%, respectively, related to those of AcMNPV. No introns were evident in the gene.