Microsatellite markers for standardized genetic management of captive colonies of rhesus macaques (Macaca mulatta)
Microsatellite markers for standardized genetic management of captive colonies of rhesus macaques (Macaca mulatta)
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DOI：
10.1002/ajp.20207
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发表时间：
2006-01-01
影响因子：
2.4
通讯作者：
Smith, DG
中科院分区：
文献类型：
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作者：
Kanthaswamy, S;Von Dollen, A;Smith, DG
在美国国立卫生研究院(NIH)赞助的每个区域研究群体中，为了保持遗传多样性并将圈养猕猴之间的遗传分化降至最低，必须确定每个群体的遗传结构。为了比较不同群体和世代的群体遗传和人口统计参数，需要一个高度信息量的遗传标记的标准小组。我们组装了一个由四个多重聚合酶链式反应(PCR)小组组成的核心标记集，其中包括15个信息含量较高的常染色体短串联重复(STR)基因座，这些基因座是从现有的、目前用于猕猴亲子鉴定和遗传管理的特征良好的标记小组中挑选出来的。然后，我们评估了这些基因座在提供个体识别和亲子鉴定的高概率以及估计对遗传管理有用的群体遗传参数方面的有效性。
To preserve genetic variability and minimize genetic subdivision among captive Macaca mulatta at each of the U.S. National Institutes of Health (NIH)-sponsored regional research colonies, the genetic structure of each colony must be characterized. To compare population genetic and demographic parameters across colonies and generations, one standard panel of highly informative genetic markers is required. We assembled a core marker set of four multiplex polymerase chain reaction (PCR) panels comprising 15 autosomal short tandem repeat (STR) loci with high information content selected from existing panels of well-characterized markers that are currently used for parentage assessment and genetic management of rhesus macaques. We then assessed the effectiveness of these loci for providing high probabilities of individual identification and parentage resolution, and for estimating population genetic parameters that are useful for genetic management.