The Ile-2041-Val mutation in the ACCase gene confers resistance to clodinafop-propargyl in American sloughgrass (Beckmannia syzigachne Steud.)
The Ile-2041-Val mutation in the ACCase gene confers resistance to clodinafop-propargyl in American sloughgrass (Beckmannia syzigachne Steud.)
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ACCase 基因中的 Ile-2041-Val 突变赋予美国腐草 (Beckmannia syzigachne Steud.) 对炔草酯的抗性
DOI：
10.1002/ps.6271
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发表时间：
2021
影响因子：
4.1
通讯作者：
Lang Pan
中科院分区：
文献类型：
--
作者：
Junzhi Wang;Yajun Peng;Wen Chen;Qin Yu;Lianyang Bai;Lang Pan
背景探索杂草抗除草剂的机制是设计抗性管理策略和合理使用除草剂的重要组成部分。丁香是我国最重要的农业杂草之一。长期使用乙酰辅酶A羧化酶（ACCase）抑制性除草剂导致B.西齐加赫内。 ACCase 抑制性除草剂包括三个化学家族：芳氧基苯氧基丙酸酯 (APP)、环己二酮 (CHD) 和苯基吡咯啉 (DEN)。 结果基于全植物剂量反应实验，aB.与易感对应物相比，丁香豆群体 (BS-R) 对 APP 除草剂精喹禾灵和炔草酯的耐药性分别是 12 倍和 20 倍，对精恶唑禾草灵、CHD 除草剂稀禾定和 PPZ 除草剂唑啉草酯的耐药性分别是 2.2 倍、2.8 倍和 2.8 倍（BS-S）。已知的细胞色素 P450 (CYP450) 抑制剂马拉硫磷和谷胱甘肽 S-转移酶 (GST) 抑制剂 4-氯-7-硝基苯并恶二唑无法逆转 BS-R 人群对炔草酯的耐药性。此外，BS-R 和 BS-S 人群之间的 CYP450 和 GST 活性没有差异。 ACCase 基因测序显示 BS-R 群体中存在 Ile-2041-Val 突变。开发了衍生的切割扩增多态性序列标记，用于快速检测特定的 Ile-2041-Val 突变。同源和异源抗性与野生型植物抗性的相关定量表明，该群体中对炔草酯的抗性是由 Ile-2041-Val 突变赋予的。结论与之前关于 2041 突变赋予的独特交叉抗性模式的报道不同，本研究表明 BS-R 群体中的 Ile-2041-Val 突变赋予对某些特定的抗性。 B.ACCase 抑制性 APP、CHD 和 PPZ 除草剂。西齐加赫内。 © 2021 中国化学工业学会
BACKGROUNDExploring the mechanisms of herbicide resistance in weeds is an important part of designing resistance management strategies and rationalizing herbicide use.Beckmannia syzigachneis one of the most important agricultural weeds in China. Long‐term use of acetyl‐CoA carboxylase (ACCase)‐inhibiting herbicides has led to the evolution of herbicide resistance inB. syzigachne. ACCase‐inhibiting herbicides comprise three chemical families: aryloxyphenoxypropionates (APPs), cyclohexanediones (CHDs) and phenylpyraxoline (DENs).RESULTSBased on whole‐plant dose–response experiments, aB. syzigachnepopulation (BS‐R) was confirmed to be 12‐ and 20‐fold resistant to the APP herbicides quizalofop‐P‐ethyl and clodinafop‐propargyl, and 2.2‐, 2.8‐ and 2.8‐fold resistant to fenoxaprop‐P‐ethyl, the CHD herbicide sethoxydim and the PPZ herbicide pinoxaden, respectively, compared with its susceptible counterpart (BS‐S). Resistance to clodinafop‐propargyl in the BS‐R population could not be reversed by the known cytochrome P450 (CYP450) inhibitor malathion and the glutathioneS‐transferase (GST) inhibitor 4‐chloro‐7‐nitrobenzoxadiazole. In addition, no difference in CYP450 and GST activity was confirmed between the BS‐R and BS‐S populations. ACCase gene sequencing revealed an Ile‐2041‐Val mutation in the BS‐R population. A derived cleaved amplified polymorphic sequence marker was developed for rapid detection of the specific Ile‐2041‐Val mutation. Correlation quantification of resistance in homo‐ and hetero‐resistantversuswild‐type plants showed that resistance to clodinafop‐propargyl in this population is conferred by the Ile‐2041‐Val mutation.CONCLUSIONUnlike previous reports on the unique cross‐resistance pattern conferred by the 2041 mutation, this study demonstrates that the Ile‐2041‐Val mutation in BS‐R population confers resistance to certain ACCase‐inhibiting APP, CHD and PPZ herbicides inB. syzigachne. © 2021 Society of Chemical Industry