Axon-regenerating retinal ganglion cells in adult rats synthesize the cell adhesion molecule L1 but not TAG-1 or SC-1
Axon-regenerating retinal ganglion cells in adult rats synthesize the cell adhesion molecule L1 but not TAG-1 or SC-1
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DOI：
10.1006/mcne.1997.0611
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发表时间：
1997-01-01
影响因子：
3.5
通讯作者：
Stuermer, CAO
中科院分区：
文献类型：
--
作者：
Jung, M;Petrausch, B;Stuermer, CAO
大鼠视网膜神经节细胞（RGCs）在PNS神经移植物存在下再生轴突。为了确定轴突再生的RGCs是否合成了它们在发育过程中所具有的细胞粘附/识别分子，对视网膜进行了L1、TAG-1和SC-1反义cRNA探针（和GAP-43进行比较）的原位杂交。用抗体检测轴突上的L1和TAG-1（以及GAP-43）蛋白。L1、TAG-1和SC-1（和Gap-43） mrna以及L1和TAG-1（和Gap-43）蛋白在胚胎、出生后和成年大鼠的RGCs中表达。视神经病变（ONL）后，存活的RGCs在ONL后2 ~ 28天继续表达L1。然而，TAG-1和SC-1的表达缺失。在移植大鼠中，轴突再生的RGCs表达L1（以及GAP-43），但不表达TAG-1和SC-1。因此，移植大鼠的轴突再生发生在L1（和GAP-43）存在的情况下，而在TAG-1和SC-1缺失的情况下。
Retinal ganglion cells (RGCs) in rats regenerate axons in the presence of a PNS nerve graft. To determine if axon-regenerating RGCs synthesize cell adhesion/recognition molecules which they possessed during development, retinae were subjected to in situ hybridization with antisense cRNA probes of L1, TAG-1, and SC-1 (and GAP-43 for comparison). L1 and TAG-1 (and GAP-43) proteins on axons were detected with antibodies. L1,TAG-1, and SC-1 (and Gap-43) mRNAs and L1 and TAG-1 (and Gap-43) proteins were expressed by RGCs in embryonic, postnatal, and adult rats. After optic nerve lesion (ONL), the surviving RGCs between 2 and 28 days after ONL continue to express L1. TAG-1 and SC-1 expression, however, is lost. In grafted rats, axon-regenerating RGCs express L1 (together with GAP-43) but neither TAG-1 nor SC-1. Thus, axonal regeneration in grafted rats occurs in the presence of L1 (and GAP-43) but in the absence of TAG-1 and SC-1.