Human monocytoid thp-1 cell line versus monocyte-derived human immature dendritic cells as in vitro models for predicting the sensitising potential of chemicals
Human monocytoid thp-1 cell line versus monocyte-derived human immature dendritic cells as in vitro models for predicting the sensitising potential of chemicals
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DOI：
10.1177/039463200702000206
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发表时间：
2007-04-01
影响因子：
3.5
通讯作者：
Burastero, S. E.
中科院分区：
文献类型：
--
作者：
Bocchietto, E.;Paolucci, C.;Burastero, S. E.
未成熟的树突状细胞（DC）在体外暴露于产生接触过敏的化学物质后调节分化标记物。 THP-1 是一种单核细胞系，保持一定的分化可塑性。在这项研究中，将人类 DC 和 THP-1 细胞作为体外模型进行比较，以预测具有不同致敏潜力的化学品的接触致敏作用。与载体相比，暴露于亚毒性浓度的强效（2,4-二硝基氯苯、DNCB 和对苯二胺，PPD）、强效（硫柳汞、TMS）、中度（四氯铂酸钠、Na2PtCl4）致敏化合物以及非致敏、刺激性十二烷基硫酸钠（SDS）后，通过流式细胞术评估 CD80 和 CD86 的表达。致敏物质（二甲基亚砜，DMSO）。在体外将 DC 和 THP-1 细胞与 DNCB、PPD、TMS 和 Na2PtCl4 一起孵育（但与 SDS 一起孵育）后，观察到 CD86 上调。在与 DNCB 和 PPD 体外孵育后，DC 上的 CD80 膜标记上调，但与 TMS、Na 2PtC14. 和 SDS 孵育后，DC 上的 CD80 膜标记上调。在 THP-1 细胞上，只有 DNCB 上调 CD80 表达。总之，细胞系 THP-1 和 DC 都是有前途的体外模型，可用于预测化学品致敏潜力的检测。 THP-1 细胞系迄今为止更容易处理，并且从实用角度来看具有相关优势。
Immature dendritic cells (DCs) modulate differentiation markers following in vitro exposure to chemicals generating contact allergies. THP-1 is a monocytoid cell line maintaining some differentiating plasticity. In this study, human DCs and THP-1 cells were compared as in vitro models to predict contact sensitisation of chemicals with different sensitising potential. Expression of CD80 and CD86 was assessed by flow cytometry after exposure to subtoxic concentrations of potent (2,4-dinitrochlorobenzene, DNCB and p-phenylendiamine, PPD), strong (thimerosal, TMS), moderate (sodium tetrachloroplatinate, Na2PtCl4) sensitising compounds as well as of non-sensitising, irritating sodium dodecyl sulphate (SDS) as compared to a vehicle of sensitising substances (dimethyl sulphoxide, DMSO). Up-regulation of CD86 following in vitro incubation of DCs and THP-1 cells with DNCB, PPD, TMS and Na2PtCl4, but not with SDS, was observed. The CD80 membrane marker was up-regulated on DCs following in vitro incubation with DNCB and PPD, but not with TMS, Na 2PtC14.and SDS. On THP-1 cells, only DNCB up-regulated CD80 expression. In conclusion, both the cell line THP-1 and DCs are promising in vitro models for assays aiming at predicting the sensitisation potential of chemicals. THP-1 cell line is by far easier to handle and offers relevant advantages from the practical point of view.