Transmission of Streptococcus mutans in a group of Turkish families
Transmission of Streptococcus mutans in a group of Turkish families
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DOI：
10.1111/j.1399-302x.2004.00163.x
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发表时间：
2004-12-01
影响因子：
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通讯作者：
Uzel, A
中科院分区：
文献类型：
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作者：
Ersin, NK;Kocabas, EH;Uzel, A
目的：采用聚合酶链式反应(AP-PCR)检测变形链球菌在一组土耳其家庭中的传播情况。方法：8名非刺激性唾液中变形链球菌水平较高的母亲和8名2~3岁的儿童参加了研究。每个孩子的菌斑样本都是用无菌牙签尖收集的，用于检测变形链球菌的数量。虽然不是最初研究设计的一部分，但变形链球菌的样本也是从同一家庭的三位父亲的未受刺激的唾液中获得的。从每个受试者的TYCSB琼脂中分离到3株典型的变形链球菌，并通过糖发酵试验进行了鉴定。以变形链球菌ATCC 10449为参比菌株。用OPA-05引物进行AP-PCR。结果：所有的母亲和父亲的孩子都有相似的基因型别。结论：母亲或父亲可能是变形链球菌传播给子女的来源。
Background/aims: To investigate the transmission of Streptococcus mutans in a group of Turkish families using AP-polymerase chain reaction (PCR) detection.Methods: Eight mothers who had high S. mutans levels in unstimulated saliva and 8 children aged between 2 and 3 years participated in the study. Plaque samples from each child were collected with the tips of sterile toothpicks for S. mutans counts. Although not part of the original study design, S. mutans samples were also obtained from the unstimulated saliva of the three fathers who shared the same households. Three typical isolates of S. mutans were isolated from TYCSB agar of each subject and identified by sugar fermentation tests. S. mutans ATCC 10449 was used as the reference strain. AP-PCR was conducted with OPA-05 primer.Results: All of the mothers and fathers shared the similar genotypes within their children. The fathers also harbored similar genotypes to their spouses.Conclusion: The mothers or the fathers could be the source for the transmission of S. mutans to their children.