Hybrid Self-Assembled Peptide Hydrogels Promote Skin Wound Healing in Diabetic Mice
Hybrid Self-Assembled Peptide Hydrogels Promote Skin Wound Healing in Diabetic Mice
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DOI：
10.1021/acsapm.3c02665
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发表时间：
2024-01
影响因子：
5
通讯作者：
Jian Li;Xiaogang Ma;Deguang Wu;Zhiwen Su;Hao Su;Zhongxun Liu;Yan Chen;Bo Yu
中科院分区：
文献类型：
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作者：
Jian Li;Xiaogang Ma;Deguang Wu;Zhiwen Su;Hao Su;Zhongxun Liu;Yan Chen;Bo Yu
糖尿病伤口的治疗非常重要，因为慢性伤口的长期不闭合对患者来说非常痛苦，甚至可能导致截肢。诱导血管化仍然是糖尿病伤口愈合的一个重大挑战。本文描述了一种基于pH敏感的自组装和共组装肽的杂化肽水凝胶。为了修复糖尿病伤口中的组织，将Ac-FKFEFKFE-QHREDGS-NH 2（F-Q）和Ac-FKFEFKFE-GRGDS-NH 2（F-G）杂交以产生水凝胶。QHREDGS来源于血管生成素-1，而GRGDS来源于骨桥蛋白。F-Q和F-G的共组装产生具有突出稳定性和刺激内皮细胞生长、粘附和迁移的能力的生物活性水凝胶（F-Q/F-G）。CD 31、bFGF和VEGF的水平在暴露于自组装功能肽水凝胶的HUVECs中显著高于对照组，表明这些因子可以促进血管生成。当将F-Q或F-G水凝胶应用于糖尿病啮齿动物的皮肤缺损区域时，刺激了血管生成和上皮再生。总之，这些发现表明F-Q/F-G水凝胶是一种具有促进表皮中伤口愈合和血管再生潜力的生物材料。
The treatment of diabetic wounds is highly important, as long-term nonclosure of chronic wounds is exceedingly painful for patients and can even lead to amputation. Inducing vascularization remains a significant challenge in diabetic wound healing. This article describes a hybrid peptide hydrogel based on pH-sensitive self-assembling and coassembling peptides. To repair tissue in diabetic wounds, Ac-FKFEFKFE-QHREDGS-NH2(F–Q) and Ac-FKFEFKFE-GRGDS-NH2(F–G) were hybridized to generate hydrogels. QHREDGS is derived from angiopoietin-1, whereas GRGDS is derived from osteopontin. Coassembly of F–Q and F–G yielded a bioactive hydrogel (F–Q/F–G) with the outstanding stability and the ability to stimulate endothelial cell growth, adhesion, and migration. The levels of CD31, bFGF, and VEGF in HUVECs exposed to the self-assembled functional peptide hydrogels were significantly greater than those in the control group, indicating that these factors could promote angiogenesis. When the F–Q or F–G hydrogel was applied to the skin defect region of diabetic rodents, angiogenesis and re-epithelialization were stimulated. In conclusion, these findings suggest that the F–Q/F–G hydrogel is a biomaterial with potential for promoting wound healing and vascular regeneration in the epidermis.