Annexin VI isoforms are differentially expressed in mammalian tissues.
Annexin VI isoforms are differentially expressed in mammalian tissues.
复制标题
膜联蛋白 VI 亚型在哺乳动物组织中差异表达。
DOI：
10.1016/0167-4889(94)90097-3
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Dedman,JR
中科院分区：
文献类型：
--
作者：
Kaetzel,MA;Pula,G;Campos,B;Uhrin,P;Horseman,N;Dedman,JR
纯化的膜联蛋白VI经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法分离后，以紧密间隔的双重链形式迁移。膜联蛋白VI在心脏中的免疫定位显示在不同的亚细胞室中染色。莫斯等人。[1]鉴定了两个cDNA，其中一个在重复结构域7的开头插入了编码VAAEIL的18个碱基。我们已经确定了小鼠Annexin VI基因的剪接位点。它包含一个由18个碱基组成的小外显子。逆转录(RT)mRNA的PCR扩增表明，在所有被测试的组织中，含有该插入片段的mRNA亚型占主导地位。制备了针对插入和缺失序列的多肽的定点抗体，并进行了亲和纯化。Annexin VI蛋白的稳态异构体比率与RT-PCR数据一致。层析实验表明，Annexin VI蛋白亚型具有生化差异。这些差异可能针对不同的亚型形成独特的细胞隔间或改变功能特性。
Purified annexin VI migrates as a closely spaced doublet when separated by SDS-PAGE. Immunolocalization of annexin VI in heart demonstrates staining at different defined subcellular compartments. Moss et al. [1] identified two cDNAs, one having an insert of 18 bases encoding VAAEIL at the beginning of repeat domain seven. We have identified the splicing site of the murine annexin VI gene. It contains a single small exon of 18 bases. PCR amplification of reverse transcribed (RT) mRNA demonstrates that, in all tissues tested, the mRNA isoform containing the insert is predominant. Site-directed antibody was produced and affinity purified against peptides reflecting the insert and deletion sequences. The steady-state isoform ratio of the annexin VI protein is consistent with the RT-PCR data. Chromatographic experiments demonstrate that the annexin VI protein isoforms have biochemical differences. These differences may target the individual isoforms to unique cellular compartments or alter functional properties.