Regulation mechanisms of retinal pigment epithelial cell migration by the TGF-β superfamily
Regulation mechanisms of retinal pigment epithelial cell migration by the TGF-β superfamily
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DOI：
10.1111/j.1395-3907.2003.00170.x
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发表时间：
2003-12-01
期刊：
影响因子：
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通讯作者：
Yamashita, H
中科院分区：
文献类型：
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作者：
Mitsuhiro, MRKH;Eguchi, S;Yamashita, H
目的：目的：探讨视网膜色素上皮细胞（retinal pigment epithelium，RPE）迁移和增殖过程中特异性受体、信号转导分子的表达及转化生长因子β（transforming growth factor-beta，TGF-β）对RPE迁移和增殖的影响。分别使用伤口愈合模型和[H-3]-胸苷掺入研究了TGF-β对迁移和增殖的影响。逆转录-聚合酶链反应（RT-CPR）检测TGF-β超家族受体和SMAD1 - 4相关RNA的表达。结果：TGF-β 4 nM和激活素A 0.36 nM刺激RPE细胞迁移。对增殖没有影响。在RPE培养物中检测到TGF-β 1型和2型受体以及SMAD 1 - 4的RNA。TGF-β刺激后，TGF-β信号转导子SMAD2从细胞质迁移到细胞核，而SMAD1则没有。结论：TGF-β可通过特定的信号转导途径调节RPE细胞迁移。
Purpose: To investigate the expression of specific receptors, signal transducers and the effect of transforming growth factor-beta(TGF-beta) on retinal pigment epithelium (RPE) migration and proliferation.Methods: Human RPE cell line D407 was used in all experiments. The effect of TGF-beta on migration and proliferation were studied using a wound healing model and [H-3]-thymidine incorporation, respectively. The expression of RNA related to the TGF-beta superfamily receptors and SMAD1-4 were assayed by reverse transcriptase-polymerase chain reaction (RT-CPR). The effects of TGF-beta on the intracellular position of SMAD were studied by immunoperoxidase and immunofluorescence.Results: Transforming growth factor-beta 4 nM and activin A 0.36 nM stimulated RPE migration. There was no effect on proliferation. RNA for TGF-beta receptors types 1 and 2, and SMAD1-4 were detected in RPE culture. Transforming growth factor-beta signal transducer SMAD2 but not SMAD1 moved from the cytoplasm to the nucleus after TGF-beta stimulation.Conclusion: Transforming growth factor-beta can regulate RPE cell migration through specific signal transduction pathways.