Characterization of the Responses of Purkinje Cells to Neurotrophin Treatment
Characterization of the Responses of Purkinje Cells to Neurotrophin Treatment
复制标题
浦肯野细胞对神经营养素治疗反应的表征
DOI：
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发表时间：
1996
影响因子：
4.7
通讯作者：
R. Alderson
中科院分区：
文献类型：
--
作者：
L. Lärkfors;R. Lindsay;R. Alderson
摘要：在来自胚胎第16天大鼠小脑的分离培养物中检查神经营养因子神经生长因子（NGF）、脑源性神经营养因子（BDNF）、神经营养因子-3（NT-3）和神经营养因子-4/5（NT-4/5）促进神经元存活和表型分化的能力。BDNF处理使神经元特异性烯醇化酶免疫阳性细胞的存活率在培养8天和10天后分别增加了250%和400%。这些神经元的一个亚群，浦肯野细胞，通过钙结合蛋白染色鉴定，在BDNF，NT-4/5或NT-3处理后增加到相同程度，约200%。通过GABA免疫反应性鉴定的GABA能神经元的数量，在脑源性神经营养因子（BDNF）处理后大大增加（470%），在NT-4/5处理后适度增加（46%），而NT-3没有影响。NGF不能增加浦肯野细胞或GABA能神经元的数量。需要在细胞接种后48小时内添加BDNF，以在8天后获得浦肯野细胞数量的最大增加。相比之下，NT-3反应几乎等同，即使在接种后延迟处理96小时。BDNF，NT-4/5和NT-3，而不是NGF，诱导立即早期基因c-fos的快速表达。使用c-fos和钙结合蛋白抗体进行免疫细胞化学双标记，以鉴定通过诱导c-fos对神经营养因子治疗作出反应的浦肯野细胞。在体外培养4天后，BDNF和NT-3都诱导钙结合蛋白免疫阳性神经元中c-fos蛋白的形成，而NT-4/5则没有。后者的结果表明，虽然BDNF和NT-4/5已被证明通过共同的受体TrkB起作用，但BDNF和NT-4/5的作用似乎并不相同。
Abstract: The ability of the neurotrophins nerve growth factor (NGF), brain‐derived neurotrophic factor (BDNF), neurotrophin‐3 (NT‐3), and neurotrophin‐4/5 (NT‐4/5) to promote neuronal survival and phenotypic differentiation was examined in dissociated cultures from embryonic day 16 rat cerebellum. BDNF treatment increased the survival of neuron‐specific enolase‐immunopositive cells by 250 and 400% after 8 and 10 days in culture, respectively. A subpopulation of these neurons, the Purkinje cells, identified by calbindin staining, was increased to an equivalent extent, ∼200%, following BDNF, NT‐4/5, or NT‐3 treatment. The number of GABAergic neurons, identified by GABA immunoreactivity, was greatly increased by treatment with BDNF (470%) and moderately by NT‐4/5 (46%), whereas NT‐3 was without effect. NGF failed to increase the number of either Purkinje cells or GABAergic neurons. Addition of BDNF within 48 h of cell plating was required to obtain a maximal increase in Purkinje cell number after 8 days. In contrast, the NT‐3 responses were nearly equivalent even if treatment was delayed for 96 h after plating. BDNF, NT‐4/5, and NT‐3, but not NGF, induced the rapid expression of the immediate early gene c‐fos. Immunocytochemical double‐labeling with antibodies to c‐fos and calbindin was used to identify Purkinje cells that responded to neurotrophin treatment by induction of c‐fos. After 4 days in vitro, both BDNF and NT‐3 induced the formation of c‐fos protein in calbindin‐immunopositive neurons, whereas NT‐4/5 did not. The latter results suggest that although BDNF and NT‐4/5 have been shown to act through a common receptor, TrkB, it appears that the effects of BDNF and NT‐4/5 are not identical.
出生后大鼠小脑神经生长因子受体 mRNA 表达的发育研究。
DOI：
10.1016/0165-3806(90)90211-g
发表时间：
1990
期刊：
Brain research. Developmental brain research
影响因子：
--
作者：
Wanaka,A;JohnsonJr,EM
通讯作者：
JohnsonJr,EM
影响因子：
10.5
作者：
BARTEL, DP;SHENG, M;GREENBERG, ME
通讯作者：
GREENBERG, ME