Endoderm induction by the organizer-secreted factors chordin and noggin in Xenopus animal caps
Endoderm induction by the organizer-secreted factors chordin and noggin in Xenopus animal caps
复制标题
DOI：
10.1002/j.1460-2075.1996.tb00832.x
复制
发表时间：
1996-09-02
期刊：
影响因子：
11.4
通讯作者：
DeRobertis, EM
中科院分区：
文献类型：
--
作者：
Sasai, Y;Lu, B;DeRobertis, EM
Spemann的组织者在原爪蟾中具有强大的神经诱导和中胚层背化活动。由于缺乏早期基因标记，第三种活性，即组织者诱导次级肠道的能力，很难通过实验分析。在这里，我们引入了内胚层基因标记内胚层蛋白，并用它来证明由组织者区域分泌的蛋白质chordin （Chd）能够诱导非洲爪蟾的内胚层分化。chd和noggin在动物帽外植体中诱导内胚层的能力受到腹化因子BMP-4的抑制。在注射chd的动物帽中，当FGF信号被显性阴性FGF受体阻断时，神经诱导被抑制，大部分外植体被诱导成为内胚层。结果表明，组织者分泌的蛋白质与已知的肽生长因子一起调节内胚层的分化。
Spemann's organizer has potent neural inducing and mesoderm dorsalizing activities in the Xenopus gastrula. A third activity, the organizer's ability to induce a secondary gut, has been difficult to analyze experimentally due to the lack of early gene markers, Here we introduce endodermin, a pan-endodermal gene marker, and use it to demonstrate that chordin (Chd), a protein secreted by the organizer region, is able to induce endodermal differentiation in Xenopus. The ability of chd, as well as that of noggin, to induce endoderm in animal cap explants is repressed by the ventralizing factor BMP-4. When FGF signaling is blocked by a dominant-negative FGF receptor in chd-injected animal caps, neural induction is inhibited and most of the explant is induced to become endoderm. The results suggest that proteins secreted by the organizer, acting together with known peptide growth factors, regulate differentiation of the endodermal germ layer.