Rapid DNA Methylation Changes after Exposure to Traffic Particles
Rapid DNA Methylation Changes after Exposure to Traffic Particles
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DOI：
10.1164/rccm.200807-1097oc
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发表时间：
2009-04-01
影响因子：
24.7
通讯作者：
Schwartz, Joel
中科院分区：
文献类型：
--
作者：
Baccarelli, Andrea;Wright, Robert O.;Schwartz, Joel
基本原理。暴露在颗粒物空气污染中与住院和死亡增加有关，特别是因心血管疾病。在与心血管疾病相关的过程中，如氧化应激、衰老和动脉粥样硬化，血液DNA甲基化含量较低。目的：我们评估了颗粒物污染是否改变了在人类基因组中具有高代表性的高度甲基化序列的DNA甲基化。方法：我们用定量聚合酶链式反应-焦磷酸测序技术检测了波士顿地区标准老龄化研究中来自718名老年参与者的1097份血液样本的DNA甲基化。我们使用协变量调整的混合模型来解释重复测量中的受试者内相关性。我们评估了周围颗粒物污染物(炭黑、空气动力学直径0.12的颗粒物)对DNA甲基化的影响。结论：我们发现暴露在交通颗粒物中后，重复元素甲基化减少。甲基化水平降低是否会导致与暴露相关的健康影响仍有待确定。
Rationale. Exposure to particulate air pollution has been related to increased hospitalization and death, particularly from cardiovascular disease. Lower blood DNA methylation content is found in processes related to cardiovascular outcomes, such as oxidative stress, aging, and atherosclerosis.Objectives: We evaluated whether particulate pollution modifies DNA methylation in heavily methylated sequences with high representation throughout the human genome.Methods: We measured DNA methylation of long interspersed nucleotide element (LINE)-1 and Alu repetitive elements by quantitative polymerase chain reaction-pyrosequencing of 1,097 blood samples from 718 elderly participants in the Boston area Normative Aging Study. We used covariate-adjusted mixed models to account for within-subject correlation in repeated measures. We estimated the effects on DNA methylation of ambient particulate pollutants (black carbon, particulate matter with aerodynamic diameter 0.12).Conclusions: We found decreased repeated-element methylation after exposure to traffic particles. Whether decreased methylation mediates exposure-related health effects remains to be determined.