IDENTIFICATION AND CHARACTERIZATION OF THE TRANSFERRIN RECEPTOR FROM NEISSERIA-MENINGITIDIS
IDENTIFICATION AND CHARACTERIZATION OF THE TRANSFERRIN RECEPTOR FROM NEISSERIA-MENINGITIDIS
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DOI：
10.1111/j.1365-2958.1988.tb00029.x
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发表时间：
1988-03-01
影响因子：
3.6
通讯作者：
MORRIS, LJ
中科院分区：
文献类型：
--
作者：
SCHRYVERS, AB;MORRIS, LJ
脑膜炎球菌转铁蛋白受体的表达（通过与过氧化物酶缀合的人转铁蛋白测定法检测）受到培养基中铁水平的调节。转铁蛋白受体经SDS-PAGE和Western印迹分析鉴定为脑膜炎奈瑟菌B16 B6中分子量为71000的铁调节外膜蛋白。铁缺乏的细胞和竞争结合实验的生长研究表明，脑膜炎球菌受体是种特异性的人转铁蛋白。相互竞争结合实验和有限的完整细胞的蛋白水解表明，转铁蛋白和乳铁蛋白是不同的。
Expression of the meningococcal transferrin receptor, detected by assay with human transferrin conjugated to peroxidase, was regulated by the level of iron in the medium. The transferrin receptor was identified by SDS-PAGE and Western blot analysis, as a 71000 molecular weight iron-regulated outer membrane protein in Neisseria meningitidis B16B6. Growth studies with iron-deficient cells and competition binding experiments demonstrated that the meningococcal receptor was species-specific for human transferrin. Reciprocal competitive binding experiments and limited proteolysis of intact cells indicated that the transferrin and lactoferrin are distinct.