Screening and quantitative determination of twelve acidic and neutral pharmaceuticals in whole blood by liquid-liquid extraction and liquid chromatography-tandem mass spectrometry.
Screening and quantitative determination of twelve acidic and neutral pharmaceuticals in whole blood by liquid-liquid extraction and liquid chromatography-tandem mass spectrometry.
复制标题
液-液萃取-液相色谱-串联质谱法筛选并定量测定全血中12种酸性和中性药物。
DOI：
10.1093/jat/34.7.367
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发表时间：
2010
影响因子：
2.5
通讯作者：
K. Linnet
中科院分区：
文献类型：
--
作者：
K. Simonsen;A. Steentoft;M. Buck;Lene Hansen;K. Linnet
我们描述了一种同时鉴定和定量全血中12种酸性和中性化合物的多方法。该方法包括简单的液-液萃取，并使用液相色谱-串联质谱法进行鉴定和定量。对水杨酸、对乙酰氨基酚、苯巴比妥、卡立普多、甲丙氨酯、托吡酯、依托度酸、氯唑沙宗、呋塞米、布洛芬、华法林和水杨酰胺的方法进行了充分验证。该方法还暂定包括硫喷妥钠、茶碱、吡罗昔康、萘普生、双氯酚酸和莫达非尼，但这些药物未包括在完整的验证程序中，此处不作详细描述。变异系数<20%的化合物的定量限为1 mg/kg，但呋塞米除外，其定量限的变异系数为32%。大多数组分的测量间隔较宽。提取效率高，反映了该方法的高产能力。
We describe a multi-method for simultaneous identification and quantification of 12 acidic and neutral compounds in whole blood. The method involves a simple liquid-liquid extraction, and the identification and quantification are performed using liquid chromatography-tandem mass spectrometry. The method was fully validated for salicylic acid, paracetamol, phenobarbital, carisoprodol, meprobamate, topiramate, etodolac, chlorzoxazone, furosemide, ibuprofen, warfarin, and salicylamide. The method also tentatively includes thiopental, theophylline, piroxicam, naproxen, diclophenac, and modafinil, but these drugs were not included in the full validation program and are not described in detail here. Limit of quantitation was 1 mg/kg for the compounds with coefficients of variation of < 20%, except for furosemide, which had a coefficient of variation of 32% at limit of quantitation. The measuring interval was wide for most components. Extraction efficiencies were high, reflecting the high-yield capacity of the method.