Anticoagulation and endothelial cell behaviors of heparin-loaded graphene oxide coating on titanium surface
Anticoagulation and endothelial cell behaviors of heparin-loaded graphene oxide coating on titanium surface
复制标题
钛表面负载肝素氧化石墨烯涂层的抗凝和内皮细胞行为
DOI：
10.1016/j.msec.2016.03.001
复制
发表时间：
2016-06-01
影响因子：
7.9
通讯作者：
Hou, Yan-Hua
中科院分区：
文献类型：
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作者：
Pan, Chang-Jiang;Pang, Li-Qun;Hou, Yan-Hua
由于其独特的物理和化学性质，氧化石墨烯在生物材料和生物医学等许多领域引起了人们的极大兴趣。本研究的目的是研究负载肝素的氧化石墨烯涂层在钛表面的内皮细胞行为和抗凝。为此，首先在钛表面覆盖聚多巴胺涂层，然后沉积氧化石墨烯涂层。最后将肝素装载在氧化石墨烯涂层上，以提高血液相容性。衰减全反射傅里叶变换红外光谱（ATR-FTIR）、拉曼光谱（Raman）和x射线光电子能谱（XPS）结果表明，在钛表面成功制备了负载肝素的氧化石墨烯涂层。扫描电镜（SEM）图像表明，在衬底上形成了由多层氧化石墨烯片组成的相对均匀的氧化石墨烯涂层。氧化石墨烯沉积后，钛表面亲水性增强，并通过负载肝素进一步显著改善。与聚多巴胺涂层和空白钛相比，氧化石墨烯涂层可以增强内皮细胞的粘附和增殖。氧化石墨烯包被上负载肝素可显著降低血小板粘附，延长活化的部分凝血活素时间（APTT），但不影响内皮细胞的粘附和增殖。因此，负载肝素的氧化石墨烯涂层可以同时增强生物材料对内皮细胞的细胞相容性和血液相容性。由于聚多巴胺涂层可以很容易地在聚合物、陶瓷和金属等大多数生物材料上制备，因此本研究的方法可能为促进血管内支架等血液接触生物医学装置的内皮化和改善血液相容性开辟了一扇新的窗口。(C) 2016 Elsevier B.V.版权所有
Owing to its unique physical and chemical properties, graphene oxide (GO) has attracted tremendous interest in many fields including biomaterials and biomedicine. The purpose of the present study is to investigate the endothelial cell behaviors and anticoagulation of heparin-loaded GO coating on the titanium surface. To this end, the titanium surface was firstly covered by the polydopamine coating followed by the deposition of the GO coating. Heparin was finally loaded on the GO coating to improve the blood compatibility. The results of attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), Raman spectroscopy and X-ray photoelectron spectroscopy (XPS) indicated that the heparin-loaded GO coating was successfully created on the titanium surface. The scanning electron microscopy (SEM) images indicated that a relative uniform GO coating consisting of multilayer GO sheets was formed on the substrate. The hydrophilicity of the titanium surface was enhanced after the deposition of GO and further improved significantly by the loading heparin. The GO coating can enhance the endothelial cell adhesion and proliferation as compared with polydopamine coating and the blank titanium. Loading heparin on the GO coating can significantly reduce the platelet adhesion and prolong the activated partial thromboplastin time (APTT) while not influence the endothelial cell adhesion and proliferation. Therefore, the heparin-loaded GO coating can simultaneously enhance the cytocompatibility to endothelial cells and blood compatibility of biomaterials. Because the polydopamine coating can be easily prepared on most of biomaterials including polymer, ceramics and metal, thus the approach of the present study may open up a new window of promising an effective and efficient way to promote endothelialization and improve the blood compatibility of blood-contact biomedical devices such as intravascular stents. (C) 2016 Elsevier B.V. All rights reserved.