In ovo treatment with CpG oligodeoxynucleotides decreases colonization of Salmonella enteriditis in broiler chickens
In ovo treatment with CpG oligodeoxynucleotides decreases colonization of Salmonella enteriditis in broiler chickens
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DOI：
10.1016/j.vetimm.2008.10.001
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发表时间：
2009-02-15
影响因子：
1.8
通讯作者：
Kogut, M. H.
中科院分区：
文献类型：
--
作者：
MacKinnon, K. M.;He, H.;Kogut, M. H.
诱导新生雏鸡的先天免疫反应对于限制细菌感染（如肠沙门氏菌血清型肠炎（SE））非常重要。CpG寡聚脱氧核苷酸（CpG- odn）可以刺激雏鸡的先天免疫反应。因此，我们研究了CpG-ODN在鸡蛋中对SE在雏鸡肠道定殖的有效性以及调节嗜异粒细胞功能的能力。从2日龄的鸡中分离嗜杂性粒细胞，用热杀SE （HK-SE）或PMA刺激氧化破裂，用HK-SE或活SE刺激脱粒。在HK-SE或PMA刺激下，CpG-ODN处理对嗜杂性细胞氧化破裂无影响。然而，与pbs处理的对照组相比，HK-SE和活SE增加了cpg - odn处理的禽类嗜杂细胞的脱颗粒（P < 0.01）。在第二个试验中，雏鸡在孵化后第10天经口感染SE， 6天后收集盲肠内容物，评估SE在肠道中的定植情况。与注射pbs的对照鸟相比，CpG-ODN治疗使SE定植减少了10倍以上（P < 0.001）。总的来说，我们首次发现在蛋中给予CpG-ODN刺激鸡嗜异源性粒细胞的先天免疫反应，并增加雏鸡对SE定殖的抗性。(C) 2008年Elsevier B.V.出版
Induction of the innate immune response in newly hatched chickens is important for limiting infections with bacteria, such as Salmonella enterica serovar Enteriditis (SE). CpG oligodeoxynucleotides (CpG-ODN) can stimulate the innate immune response of young chickens. Therefore, we examined the effectiveness of CpG-ODN administered in ovo on intestinal colonization by SE and the ability to modulate the function of heterophils in young chickens. Heterophils were isolated from 2-day-old chickens and were stimulated with heat-killed SE (HK-SE) or PMA for oxidative burst and HK-SE or live SE for degranulation assays. CpG-ODN treatment had no effect on heterophil oxidative burst when stimulated with HK-SE or PMA. However, HK-SE and live SE increased degranulation (P < 0.01) in heterophils from CpG-ODN-treated birds compared to PBS-treated controls. In a second experiment, chickens were orally infected with SE on day 10 post-hatch and cecal contents were collected 6 days later for assessment of SE intestinal colonization. CpG-ODN treatment reduced SE colonization by greater than 10-fold (P < 0.001) compared to PBS-injected control birds. Overall, we show for the first time that CpG-ODN given in ovo stimulates innate immune responsiveness of chicken heterophils and increases resistance of young chickens to SE colonization. (C) 2008 Published by Elsevier B.V.