Positions in the 30S ribosomal subunit proximal to the 790 loop as determined by phenanthroline cleavage.
Positions in the 30S ribosomal subunit proximal to the 790 loop as determined by phenanthroline cleavage.
复制标题
通过菲咯啉裂解确定 30S 核糖体亚基中靠近 790 环的位置。
DOI：
10.1017/s1355838299990143
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Hill,WE
中科院分区：
文献类型：
--
作者：
Muth,GW;Hennelly,SP;Hill,WE
将rRNA定位在核糖体内仍然是一个具有挑战性的问题。这种定位对于理解核糖体功能至关重要，因为各种rRNA区域相互作用以形成配体（如tRNA和mRNA）的合适结合位点。我们已经使用了菲咯啉，化学核酸酶，作为邻近探针，以帮助阐明的区域rRNA的茎环结构的近邻集中在核苷酸790在16 S rRNA的大肠杆菌30 S核糖体亚基。使用共价连接到与nt 787-795互补的DNA寡聚体上的邻菲咯啉，我们发现nt 582-584、693-694、787-790和795-797被强烈切割，并且必须位于DNA寡聚体5′端拴系位点的约15 μ m范围内，该位点与16 S rRNA的nt 795相邻。
Positioning rRNA within the ribosome remains a challenging problem. Such positioning is critical to understanding ribosome function, as various rRNA regions interact to form suitable binding sites for ligands, such as tRNA and mRNA. We have used phenanthroline, a chemical nuclease, as a proximity probe, to help elucidate the regions of rRNA that are near neighbors of the stem-loop structure centering at nt 790 in the 16S rRNA of the Escherichia coli 30S ribosomal subunit. Using phenanthroline covalently attached to a DNA oligomer complementary to nt 787–795, we found that nt 582–584, 693–694, 787–790, and 795–797 were cleaved robustly and must lie within about 15 Å of the tethered site at the 5′ end of the DNA oligomer, which is adjacent to nt 795 of 16S rRNA.