Preparationo of Rubst Metal-Free Magnetic Nanoemulsiions Encapsulating Low-Molecular-Weight Nitoxide Radicals and Hydrophobic Drugs Directed Toward MRI-Visible Targeted Delivery.
Preparationo of Rubst Metal-Free Magnetic Nanoemulsiions Encapsulating Low-Molecular-Weight Nitoxide Radicals and Hydrophobic Drugs Directed Toward MRI-Visible Targeted Delivery.
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封装低分子量氮氧化物自由基和疏水性药物的无锈金属磁性纳米乳液的制备，用于 MRI 可见靶向递送。
DOI：
10.1002/chem.201702785
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
R.
中科院分区：
文献类型：
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作者：
Nagura;K.;Takemoto;Y.;Moronaga;S.;Uchida;Y.;Shimono;S.;Shiino;A.;Tanigaki;K.;Amano;T.;Yoshino;F.;Noda;Y.;Koizumi;S.;Komatsu;N.;Kato;T.;Yamauchi;J.;Tamura;R.
为了开发用于通过磁共振（MR）成像可见的靶向药物递送系统的治疗诊断纳米药物，在pH 7.4磷酸盐缓冲盐水（PBS）中制备了由生物相容性表面活性剂和疏水性低分子量2，2，5_三甲基-5-（4-烷氧基）苯基吡咯烷-N-氧基自由基组成的坚固的无金属磁性纳米乳液（平均粒径小于20 nm）。 纳米乳液的结构通过电子顺磁共振光谱、动态光散射和小角中子散射测量来表征。纳米乳液显示出高胶体稳定性，低细胞毒性，足够的还原阻力过量的抗坏血酸，和足够的对比度增强在PBS中的质子纵向弛豫时间（T1）加权MR图像在体外（和初步在体内）。此外，疏水性抗癌药物紫杉醇可以包封在纳米颗粒内，并且所得的紫杉醇负载纳米乳液有效地掺入HeLa细胞中以抑制细胞生长。
With a view to developing a theranostic nanomedicine for targeted drug delivery systems visible by magnetic resonance (MR) imaging, robust metal‐free magnetic nanoemulsions (mean particle size less than 20 nm) consisting of a biocompatible surfactant and hydrophobic, low molecular weight 2,2,5‐trimethyl‐5‐(4‐alkoxy)phenylpyrrolidine‐N‐oxyl radicals were prepared in pH 7.4 phosphate‐buffered saline (PBS). The structure of the nanoemulsions was characterized by electron paramagnetic resonance spectroscopy, and dynamic light scattering and small‐angle neutron‐scattering measurements. The nanoemulsions showed high colloidal stability, low cytotoxicity, enough reduction resistance to excess ascorbic acid, and sufficient contrast enhancement in the proton longitudinal relaxation time (T1) weighted MR images in PBS in vitro (and preliminarily in vivo). Furthermore, the hydrophobic anticancer drug paclitaxel could be encapsulated inside the nanoparticles, and the resulting paclitaxel‐loaded nanoemulsions were efficiently incorporated into HeLa cells to suppress cell growth.