Sequence characterization of an unusual lysozyme gene expressed in the intestinal tract of the reduviid bug Triatoma infestans (Insecta)
Sequence characterization of an unusual lysozyme gene expressed in the intestinal tract of the reduviid bug Triatoma infestans (Insecta)
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DOI：
10.1007/s00436-007-0751-0
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发表时间：
2008-01-01
影响因子：
2
通讯作者：
Schaub, G. A.
中科院分区：
文献类型：
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作者：
Balczun, C.;Knorr, E.;Schaub, G. A.
溶菌酶等抗菌蛋白是昆虫对细菌非特异性免疫反应的重要组成部分。利用PCR和cDNA末端快速扩增技术，从致病锥虫（Triatoma infestans）中扩增出一种新的溶菌酶基因，命名为Lysozyme2。该基因在昆虫的小肠中表达。推导的蛋白质序列显示高达70%的相似性从其他物种的溶菌酶。此外，该蛋白质与其他昆虫溶菌酶具有显著的结构一致性。溶菌酶2蛋白的一个显著特征是经典C型溶菌酶活性位点的保守氨基酸残基谷氨酸和天冬氨酸被缬氨酸和酪氨酸取代。
Antibacterial proteins like lysozyme are important components of the insect non-specific immune response against bacteria. The complementary deoxyribonucleic acid (cDNA) encoding a new lysozyme from Triatoma infestans, named lysozyme2, has been amplified by polymerase chain reaction and the rapid amplification of cDNA ends technique. The gene is expressed in the small intestine of the insect. The deduced protein sequence shows up to 70% similarity to lysozymes from other species. Furthermore, the protein exhibits significant structural concordance to other insect lysozymes. A striking feature of the lysozyme2 protein is the replacement of the conserved amino acid residues of the active site of classical c-type lysozymes, glutamate and aspartate, by valine and tyrosine.