Effects of transforming growth factor β1 and dexamethasone on the growth and chondrogenic differentiation of adipose-derived stromal cells
Effects of transforming growth factor β1 and dexamethasone on the growth and chondrogenic differentiation of adipose-derived stromal cells
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DOI：
10.1089/10763270360728215
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发表时间：
2003-12-01
影响因子：
--
通讯作者：
Guilak, F
中科院分区：
文献类型：
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作者：
Awad, HA;Halvorsen, YDC;Guilak, F
研究了通常用于在不同细胞培养系统中诱导软骨形成分化的可溶性介质和培养基补充剂的作用，以确定其剂量反应曲线以及对脂肪源性成体基质（ADAS）细胞软骨形成的潜在协同作用。将人 ADAS 细胞悬浮在藻酸盐珠中，在含有胰岛素、转铁蛋白和亚硒酸 (ITS+) 或胎牛血清 (FBS) 的基础培养基中培养，并用不同剂量和组合的 TGF-β1（0、1 和 10 ng/mL）和地塞米松（0、10 和 100 nM）进行处理。通过测量DNA含量、蛋白质和蛋白多糖合成率以及蛋白多糖积累来评估细胞生长和软骨形成分化。 ITS+ 和 TGF-β1 的组合显着增加了细胞增殖。在 ITS+ 或 FBS 存在的情况下，TGF-β1 和地塞米松可提高蛋白质合成率。虽然在 FBS 存在的情况下，TGF-β1 显着增加了蛋白聚糖的合成和积累 1.5 至 2 倍，但这种作用被地塞米松抑制。总之，TGF-β1 和 ITS+ 的组合刺激了人类 ADAS 细胞的细胞生长以及蛋白质和蛋白聚糖的合成。添加地塞米松似乎可以增强蛋白质合成，但对蛋白聚糖合成和积累具有抑制作用。
The effects of soluble mediators and medium supplements commonly used to induce chondrogenic differentiation in different cell culture systems were investigated to define their dose-response profiles and potentially synergistic effects on the chondrogenic differentiation of adipose-derived adult stromal (ADAS) cells. Human ADAS cells were suspended within alginate beads and cultured in basal medium with insulin, transferrin, and selenious acid (ITS+) or fetal bovine serum (FBS) and treated with different doses and combinations of TGF-beta1 (0, 1, and 10 ng/mL) and dexamethasone (0, 10, and 100 nM). Cell growth and chondrogenic differentiation were assessed by measuring DNA content, protein and proteoglycan synthesis rates, and proteoglycan accumulation. The combination of ITS+ and TGF-beta1 significantly increased cell proliferation. Protein synthesis rates were increased by TGF-beta1 and dexamethasone in the presence of ITS+ or FBS. While TGF-beta1 significantly increased proteoglycan synthesis and accumulation by 1.5- to 2-fold in the presence of FBS, such effects were suppressed by dexamethasone. In summary, the combination of TGF-beta1 and ITS+ stimulated cell growth and synthesis of proteins and proteoglycans by human ADAS cells. The addition of dexamethasone appeared to amplify protein synthesis but had suppressive effects on proteoglycan synthesis and accumulation.