Isolation, sequencing and expression of the gene encoding a major protein from the backteriophage associated with Bartonella henselae
Isolation, sequencing and expression of the gene encoding a major protein from the backteriophage associated with Bartonella henselae
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DOI：
10.1016/s0378-1119(97)00580-5
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发表时间：
1998-01-05
期刊：
影响因子：
3.5
通讯作者：
Anderson, B
中科院分区：
文献类型：
--
作者：
Bowers, TJ;Sweger, D;Anderson, B
克隆并测序了编码与汉赛巴尔通体中所含噬菌体相关的31 kDa主要蛋白（Pap31）的基因。对所得序列的分析揭示了编码279个氨基酸的蛋白质的837个核苷酸的开放阅读框架。然后将pap31亚克隆到pUC 19中lacZ启动子的下游。采用聚合酶链反应扩增pap31基因，以线性扩增子为模板进行体外转录和翻译。由该反应合成了表观分子量约为31 kDa的蛋白质。在分析推导的氨基酸序列，一个潜在的信号序列和一个共识信号肽酶切割位点被确定，表明Pap31的修饰后，和成熟的蛋白质可能被靶向到宿主膜。(C)1997年Elsevier Science B.V.
The gene encoding a 31-kDa major protein (Pap31) associated with the bacteriophage harbored in Bartonella henselae was cloned and sequenced. Analysis of the resulting sequence revealed an open reading frame of 837 nucleotides coding for a protein of 279 amino acids. pap31 was then subcloned downstream of the lacZ promoter in pUC19. pap31 was amplified by polymerase chain reaction, and the linear amplicon was used as template for in-vitro transcription and translation. A protein with an apparent molecular mass of approximately 31 kDa was synthesized from this reaction. Upon analysis of the deduced aa sequence, a potential signal sequence and a consensus signal peptidase cleavage site were identified, indicative that Pap31 is modified posttranslationally, and the mature protein may be targeted to the host membrane. (C) 1997 Elsevier Science B.V.