Detection of levodopa, dopamine and its metabolites in rat striatum dialysates following peripheral administration of L-DOPA prodrugs by mean of HPLC-EC
Detection of levodopa, dopamine and its metabolites in rat striatum dialysates following peripheral administration of L-DOPA prodrugs by mean of HPLC-EC
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DOI：
10.1016/j.jpba.2004.09.029
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发表时间：
2005-01-04
影响因子：
3.4
通讯作者：
Parenti, C
中科院分区：
文献类型：
--
作者：
Cannazza, G;Di Stefano, A;Parenti, C
建立了高效液相色谱（HPLC）方法，同时检测口服左旋多巴或其前药后大鼠纹状体扩张液中左旋二羟基苯丙氨酸（L-DOPA）、多巴胺（DA）、二羟基苯乙酸（DOPAC）和高香草酸（HVA）的含量。色谱系统使用反相 C-IS 柱，电化学检测电压为 +0.30 V。流动相由 0.05 M 柠檬酸组成。 EDTA 50 muM、辛基磺酸钠 0.4 nM（pH 2.9）和 8%、甲醇（v/v），流速 1 ml/min。校准曲线在 10 nm 至 100 muM 的浓度范围内呈线性，L-DOPA 的检测下限为 125 fmol。信号噪声与比率为 3 时，DOPAC 为 50 fmol，DA 为 250 fmol，HVA 为 150 fmol。重复性（或日内精度），以相对标准偏差表示。均优于 4%。已经描述了微透析探针的构造。每个微透析探针的体外相对回收率是。评估结果表明，对于 CV 为 1% 至 4% 的所有分析物，它们是相似的且可重复。采用HPLC-EC法检测细胞外L-DOPA的水平。 DA。口服六种新的潜在 L-DOPA 前药后自由活动大鼠纹状体透析液中的 DOPAC 和 HVA。 (C) 2004 Elsevier B.V. 保留所有权利。
A high performance liquid chromatography (HPLC) method was developed to detected simultaneously L-dihydroxyphenylalanine (L-DOPA), dopamine (DA), dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) in rat striatum dilaysates following oral administration of L-DOPA or its prodrugs. The chromatographic system uses a reversed-phase C-IS column with electrochemical detection at +0.30 V. Mobile phase consisted of 0.05 M citric acid. sodium EDTA 50 muM, sodium octylsulphonate 0.4 nM at pH of 2.9 and 8%, methanol (v/v) at a flow rate of 1 ml/min. The calibration curves were linear over the concentration range of 10 nm to 100 muM and the lower limits of detections were 125 fmol for L-DOPA. 50 fmol for DOPAC, 250 fmol for DA and 150 fmol for HVA at signal noise to ratio of 3. The repeatability (or intra-day precision), expressed by the relative standard deviation. were better than 4%. The construction of microdialysis probes has been described. The in vitro relative recoveries of each microdialysis probe were. evaluated and the results show that they are similar and reproducible for all the analytes with CVs from 1 to 4%. The HPLC-EC method was applied to detect the extracellular levels Of L-DOPA. DA. DOPAC and HVA in the striatum dialysates of freely moving rats after oral administration of six new potential L-DOPA prodrugs. (C) 2004 Elsevier B.V. All rights reserved.