Recombinant HIV-1 nucleocapsid protein accelerates HIV-1 reverse transcriptase catalyzed DNA strand transfer reactions and modulates RNase H activity.
Recombinant HIV-1 nucleocapsid protein accelerates HIV-1 reverse transcriptase catalyzed DNA strand transfer reactions and modulates RNase H activity.
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DOI：
10.1021/bi00250a036
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发表时间：
1994-11
期刊：
影响因子：
2.9
通讯作者：
J. A. Peliska;Shankar Balasubramanian;D. Giedroc;Stephen J. Benkovic
中科院分区：
文献类型：
--
作者：
J. A. Peliska;Shankar Balasubramanian;D. Giedroc;Stephen J. Benkovic
用动力学方法研究了人类免疫缺陷病毒1型(HIV-1)重组核衣壳蛋白(NCp7)对HIV-1逆转录酶(HIV-1 RT)催化的DNA链转移反应的影响。NCp7被证明同时调节DNA链转移合成的速度和效率。有证据表明，NCp7在链转移过程中催化新生DNA中间体和RNA受体模板的退火中起到了作用。NCp7还能增强HIV-1RT的核糖核酸酶H活性，改变RNA水解的特异性，提示NCp7在HIV-1RT催化的链转移中起直接作用。这些发现对逆转录病毒逆转录的意义进行了讨论。
The effect of recombinant nucleocapsid protein (NCp7) from human immunodeficiency virus type 1 (HIV-1) on HIV-1 reverse transcriptase (HIV-1 RT) catalyzed DNA strand transfer reactions has been studied using kinetic methods with a defined template--primer model system. NCp7 is shown to modulate both the rate and the efficiency of DNA strand transfer synthesis. Evidence is presented that supports the role of NCp7 in catalyzing the annealing of a nascent DNA intermediate and RNA acceptor template during strand transfer. NCp7 was also found to enhance the ribonuclease H activity of HIV-1 RT and change the specificity of RNA hydrolysis, suggesting a direct role of NCp7 in HIV-1 RT catalyzed strand transfer. The implications of these findings for retroviral reverse transcription are addressed.