In vitro allelopathic effects of compounds from Cerbera manghas L. on three Dinophyta species responsible for harmful common red tides
In vitro allelopathic effects of compounds from Cerbera manghas L. on three Dinophyta species responsible for harmful common red tides
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Cerbera manghas L. 化合物对造成有害常见赤潮的三种甲藻的体外化感作用
DOI：
10.1016/j.scitotenv.2020.142253
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发表时间：
2021-02-01
影响因子：
9.8
通讯作者：
Duan, Shunshan
中科院分区：
文献类型：
--
作者：
Chen, Qi;Sun, Dong;Duan, Shunshan
化感作用被认为是控制有害藻华(HABS)的一种经济和生态友好的方法，因为化感物质比传统的杀藻剂更容易降解，造成的污染更小。我们首先考察了传统药用植物芒果对臭名昭著的塔玛亚历山大藻、锥状斯氏杆菌和米氏卡氏杆菌的抑制作用。然后，我们用UPLC-MS对潜在的杀藻化合物进行了鉴定和定量，并测定了它们的活性。水提物对塔玛、锥状链霉菌和三叶藻的EC_(50)-120HAT分别为0.986、1.567和1.827克L-1。在原液中检出3种潜在的化感物质：奎尼酸(QA)(28.81mgL-1)、原儿茶酸(PA)(53.91mgL-1)和连翘苷(Pd)(26.17mgL-1)。结果表明：1)QA对藻类无抑制作用；2)PA对藻类有中等毒性(EC50-120h，对塔玛螺旋藻、锥形螺旋藻和三叶藻的EC50-120h分别为0.22、0.28和0.35 mM)；这些结果表明，PA可能是所研究藻类水浸液中的主要化感物质。此外，PA还可能通过抑制苯二酚电子的还原，破坏PSII中的电子传递，从而对蝴蝶兰的光合作用产生负面影响。总之，这是第一次对芒果中的化感物质进行量化的研究。此外，芒果和原儿茶酸有可能成为控制和减轻由甲藻引起的赤潮的杀藻剂。(C)2020爱思唯尔B.V.保留所有权利。
Allelopathy is regarded as an economic and eco-friendly approach for the control of harmful algal blooms (HABs) because allelochemicals degrade easily and cause less pollution than traditional algicides. We first surveyed the inhibitory effect of the traditional medicinal plant Cerbera manghas L. on the notorious dinoflagellates Alexandrium tamarense, Scrippsiella trochoidea, and Karenia mikimotoi. Then, we identified and quantified the potential algicidal compounds by UPLC-MS and determined their activity. The aqueous extract inhibited algae with EC50-120 hat 0.986,1.567 and 1.827 g L-1 for A. tamarense, S. trochoidea, and K. mikimotoi, respectively. Three potential allelochemicals were quantified in the stock solution: quinic acid (QA) (28.81 mg L-1), protocatechuic acid (PA) (53.91 mg L-1), and phloridzin (PD) (26.17 mg L-1). Our results illustrated that 1) QA did not have an inhibitory effect, 2) PA had medium toxicity to algae (EC50-120h: 0.22, 0.28, and 0.35 mM for A. tamarense, S. trochoidea, and K. mikimotoi), and 3) PD had low toxicity (EC50-120h > 0.66 mM). These findings suggested that PA might be the main allelopathic compound in the aqueous extract of the studied algae. In addition, PA could have a negative effect on the photosynthesis of S. trochoidea by impeding the reduction of quinone electrons and destroying electron transfer in PSII. In summary, this was the first study to quantify allelochemicals in C. manghas fruit. Moreover, C. manghas and protocatechuic have the potential to be algicides to control and mitigate the HABs caused by dinoflagellates. (C) 2020 Elsevier B.V. All rights reserved.