Differential biological effects in two pedigrees of clam Ruditapes philippinarum exposed to cadmium using iTRAQ-based proteomics
Differential biological effects in two pedigrees of clam Ruditapes philippinarum exposed to cadmium using iTRAQ-based proteomics
复制标题
使用基于 iTRAQ 的蛋白质组学研究暴露于镉的菲律宾蛤仔两个家系的差异生物效应
DOI：
10.1016/j.etap.2018.12.002
复制
发表时间：
2019-01-01
影响因子：
4.3
通讯作者：
Wu, Huifeng
中科院分区：
文献类型：
--
作者：
Lu, Zhen;Wang, Shuang;Wu, Huifeng
由于工业排放的影响，镉（Cd）已成为渤海典型的重金属污染物之一。菲律宾蛤仔（Ruditapes philippinarwn）是一种常见的污染生物监测资源，其主要类群为白色蛤和斑蛤。然而，对重金属在不同文蛤家系中的生物学效应差异研究较少。在这项工作中，蛋白质组分析进行揭示差异蛋白质组学反应的白色和斑马蛤镉暴露（200 μ g/L）48小时，随后进行生物信息学分析。蛋白质组学研究表明，镉处理诱导更多的差异表达蛋白（DEPs）的白色蛤样品比斑马蛤样品。基于DEPs的分析，我们发现两个家系的文蛤都参与了一些重要的生物学过程，包括免疫应答和代谢。独特的是，镉处理的白色蛤样品中的细胞信号转导，蛋白质水解和能量生产相关的一些过程得到了增强。相比之下，在一些独特的蛋白质水解和能量生产过程中的消耗引起镉处理的斑马蛤样品，以及在基因表达的障碍。此外，Cd暴露还导致白色文蛤体内CAT和POD活性升高，而斑马蛤体内SOD和CAT活性降低，这与蛋白质组学反应一致。总体而言，这些结果证实了不同的生物学效应的白色和斑马蛤镉处理，建议动物的系谱应考虑在生态毒理学研究。
Due to the industrial discharges, cadmium (Cd) has been one of typical heavy metal pollutants in the Bohai Sea. Manila clam Ruditapes philippinarwn is frequently used for pollution biomonitoring and consists of several pedigrees, of which White and Zebra clams are the dominant pedigrees along the Bohai Sea coast. However, limited attention has been paid on the differential biological effects in different pedigrees of clam to heavy metals. In this work, the proteome profiling analysis was performed to reveal the differential proteomic responses in White and Zebra clams to Cd exposure (200 mu g/L) for 48 h, followed by bioinformatical analysis. The proteomic investigations showed that Cd treatment induced more differentially expressed proteins (DEPs) in White clam samples than in Zebra clam samples. Based on the DEPs, we found that some key biological processes consisting of immune response and metabolism were commonly induced in both two pedigrees of clam. Uniquely, some processes related to cellular signaling, proteolysis and energy production were enhanced in Cd-treated White clam samples. Comparatively, the depletion in some unique processes on proteolysis and energy production was elicited in Cd-treated Zebra clam samples, as well as disorder in gene expression. Moreover, Cd exposure caused increases in CAT and POD activities in White clam samples and decreases in SOD and CAT activities in Zebra clams samples, which were consistent with the proteomic responses. Overall, these findings confirmed the differential biological effects of White and Zebra clams to Cd treatment, suggesting that the pedigree of animal should be taken into consideration in ecotoxicology studies.