A probe with double acetoxyl moieties for hydrazine and its application in living cells.
A probe with double acetoxyl moieties for hydrazine and its application in living cells.
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DOI：
10.1016/j.saa.2018.05.112
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发表时间：
2018-10
期刊：
影响因子：
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通讯作者：
Xinrong Shi;C. Yin;Ying Wen;Yongbin Zhang;Fangjun Huo
中科院分区：
文献类型：
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作者：
Xinrong Shi;C. Yin;Ying Wen;Yongbin Zhang;Fangjun Huo
联氨作为一种常见的化学还原剂，在各个领域得到了广泛的应用。然而，其对人类和环境的高毒性也引起了人们的关注。在本工作中，我们成功地合成了一种新的基于香豆素的联氨“开启”荧光探针。该探针以双乙酰氧基为反应部位，对蒸馏水中肼的检测下限可达2.98 nM，低于美国环保局的标准(10 ppb)。此外，与其他分子相比，它对联氨具有明显的荧光增强作用和高选择性。此外，该探针可以显示活细胞中的联氨。
As a common chemical reductant, hydrazine has been widely used in various fields. However, its high toxicity to human and environment have also attracted people's attention. In this work, a new fluorescence “turn-on” probe based on coumarin for hydrazine was successfully synthesized. The probe with double acetoxyl moieties as the reaction sites can obtain the detection limit as low as 2.98 nM for the detection of hydrazine in distilled water, which was lower than the U.S. Environmental Protection Agency standard (10 ppb). In addition, it also responded obvious fluorescence enhancement and high selectivity to hydrazine over other molecules. Furthermore, this probe could visualize the hydrazine in living cells.