Marinobacter aromaticivorans sp nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from sea sediment
Marinobacter aromaticivorans sp nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from sea sediment
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Marinobacteraromaticivorans sp nov.，一种从海洋沉积物中分离出来的多环芳烃降解细菌
DOI：
10.1099/ijsem.0.000722
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发表时间：
2016-01-01
影响因子：
2.8
通讯作者：
Zheng, Li
中科院分区：
文献类型：
--
作者：
Cui, Zhisong;Gao, Wei;Zheng, Li
从南海沉积物中分离到一株革兰氏染色阴性、略耐盐的杆状细菌，命名为D15- 8 P（T）。该菌株可以在0.5%至10%（w/v）（最佳0.5- 1.5%）的NaCl浓度范围内生长，并且可以在10-40 ° C（最佳25 ° C）和pH 5.5-9.5（最佳pH 7.0-8.0）下培养。该菌株对过氧化氢酶、氧化酶和吐温80的水解呈阳性，但对DNA和明胶的水解、亚硝酸盐还原、吲哚产生、Voges-Proskauer反应和甲基红试验呈阴性。菌株D15- 8 P（T）对萘、菲和蒽具有较好的降解能力。主要呼吸醌是Q-9。主要的细胞脂肪酸为C-12：0（11.5%）、C-14：0 3-甲基（22.0%）、C-16：0（19.2%）、C-16：1 omega 9 c（22.9%）和C-18：1 omega 9 c（6.7%）。极性脂质为磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油、一种未鉴别的氨基磷脂和一种未鉴别的磷脂。DNA G + C含量为56.8mol%。基于16 S rRNA基因的系统发育分析表明，菌株D15- 8 P（T）与Marinamaritimus JCM 12521（T）的亲缘关系最近。（16 S rRNA基因序列相似性为98.5%），海洋微藻（Marinobacteriancticus）CGMCC 1.10835（T）（98.1%）、解脂海蛞蝓DSM 15157（T）（97.1%）和几内亚海蛞蝓CECT 7243（T）（97.0%）。gyrB基因分析和DNA-DNA杂交的结果均低于临界值（gyrB基因序列相似性为90%，DNA-DNA杂交为70%）。根据多相分类学研究结果，菌株D15- 8 P（T）代表了海蛞蝓属的一个新种，并建议将其命名为Marinalumaromaticivorans sp. nov.。模式菌株为D15- 8 P（T）（=CGMCC 1.11015（T）=KCTC 23781（T））。
A rod-shaped, Gram-stain-negative, slightly halotolerant bacterium, designated strain D15-8P(T), was isolated from a sediment sample from the South China Sea. The strain could grow in NaCl concentrations ranging from 0.5 % to 10 % (w/v) (optimum 0.5-1.5 %), and could be cultivated at 10-40 degrees C (optimum 25 degrees C) and pH 5.5-9.5 (optimum pH 7.0-8.0). The strain was positive for catalase, oxidase, and hydrolysis of Tween 80, but negative for hydrolysis of DNA and gelatin, nitrite reduction, indole production, Voges-Proskauer reaction, and methyl red test. Strain D15-8P(T) could biodegrade naphthalene, phenanthrene, and anthracene. The major respiratory quinone was Q-9. The main cellular fatty acids were C-12 : 0 (11.5 %), C-14 : 0 3-methyl (22.0 %), C-16 : 0 (19.2 %), C-16 : 1 omega 9c (22.9 %), and C-18 : 1 omega 9c (6.7 %). The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified aminophospholipid and an unidentified phospholipid. The DNA G + C content was 56.8 mol%. Phylogenetic analyses based on 16S rRNA genes showed that strain D15-8P(T) was most closely related to Marinobacter maritimus JCM 12521(T) (98.5 % 16S rRNA gene sequence similarity), Marinobacter antarcticus CGMCC 1.10835(T) (98.1 %), Marinobacter lipolyticus DSM 15157(T) (97.1 %), and Marinobacter guineae CECT 7243(T) (97.0 %). Results of the gyrB gene analysis and DNA-DNA hybridization were both less than the cut-off values (90 % for gyrB gene sequence similarity and 70 % for DNA-DNA hybridization). On the basis of this taxonomic study using a polyphasic approach, strain D15-8P(T) represents a novel species of the genus Marinobacter, for which the name Marinobacter aromaticivorans sp. nov. is proposed. The type strain is D15-8P(T) (=CGMCC 1.11015(T) =KCTC 23781(T)).