Short-term tumor cell lines from breast cancer for use as autologous tumor cell vaccines in the treatment of breast cancer
Short-term tumor cell lines from breast cancer for use as autologous tumor cell vaccines in the treatment of breast cancer
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DOI：
10.1089/10849780152389393
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发表时间：
2001-06-01
影响因子：
3.4
通讯作者：
Nayak, SK
中科院分区：
文献类型：
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作者：
Dillman, RO;Beutel, LD;Nayak, SK
目的：我们试图建立乳腺癌患者自体肿瘤的短期培养，以用于乳腺癌切除后的主动特异性免疫治疗(即自体疫苗)和/或转移瘤的治疗。方法：从1990年10月至1999年12月，霍格癌症中心细胞生物学实验室试图从115例乳腺癌标本中建立短期肿瘤细胞系，这些标本来自56个乳腺癌原发灶、17个腋窝淋巴结、14个其他淋巴结/软组织部位、10个胸壁复发和6个恶性胸腔积液。成功的定义是5×10(7)活细胞的生长，提交的组织的组织学、细胞形态和抗原表型证实了其恶性本质和乳腺癌起源。结果：115例标本中仅有8例(7%)扩增至5×10(7)细胞，其中2例来自胸壁复发，1例来自锁骨上结节、1例脐结节、1例肝、1例大网膜和胸水。其中两个成功的细胞系是从最初冷冻保存的组织建立的；另两个是从新鲜肿瘤开始的。区域/远处转移的成功率7/55(13%)高于原发肿瘤1/56(1.8%)(p=0.063)。在霍格医院采集的肿瘤的成功率为4/97(4%)，而来自远处的肿瘤成功率为4/14(31%)，但除一例以外，所有来自远处的肿瘤都是转移性病变。从大小不等的转移灶中成功地建立了肿瘤细胞系
Objective: We tried to establish short-term cultures of autologous tumors from patients with breast carcinoma for potential use as active specific immunotherapy (i.e., autologous vaccine) after resection of primary breast cancer, and/or for the treatment of metastases. Methods: Between 10/90 and 12/99 the cell biology laboratory of the Hoag Cancer Center attempted to establish short-term tumor cell lines from 115 breast cancer specimens from 56 primary breast lesions, 17 axillary nodes, 14 other lymph node/soft tissue sites, 10 chest wall recurrences, and 6 thoracenteses of malignant pleural effusions. Success was defined by growth of 5 X 10(7) viable cells whose malignant nature and breast cancer origin was confirmed by histology of the submitted tissue, cell morphology and antigenic phenotyping. Variables associated with successful growth of short-term cell lines were examined Results: Expansion to 5 X 10(7) cells was achieved for only 8/115 samples [7%] including two from chest wall recurrences, and one each from a supraclavicular node, an umbilical node, liver, omentum, and pleural fluid. Two of the successful cell lines were established from tissue that originally had been cryopreserved; the others were initiated from fresh tumor. The success rate was better from regional/distant metastases 7/55 (13%) compared to primary tumors 1/56 (1.8%) (p = 0.063). The success rate for tumors harvested at Hoag Hospital was 4/97 (4%) compared to 4/14 from (31%) distant sites, but all but one of the tumors from a distant geographic site was a metastatic lesion. Tumor cell lines were successfully established from metastatic lesions ranging in size from