Development of murine hepatic sinusoidal endothelial cells characterized by the expression of hyaluronan receptors
Development of murine hepatic sinusoidal endothelial cells characterized by the expression of hyaluronan receptors
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DOI：
10.1002/dvdy.21227
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发表时间：
2007-08-01
影响因子：
2.5
通讯作者：
Miyajima, Atsushi
中科院分区：
文献类型：
--
作者：
Nonaka, Hidenori;Tanaka, Minoru;Miyajima, Atsushi
内皮细胞（ECs）显示不同的结构和功能特征，这取决于组织和发育阶段;然而，组织特异性ECs的发展仍然知之甚少。在这里，我们描述了发展的基础上表达的透明质酸受体Stab 2和Lyve-1的小鼠肝窦。肝芽内和周围的Flk- 1（+）细胞开始表达Stab 2，在胚胎（E）9.5天，在血管腔形成之前。肝窦内皮细胞（HSEC）开始表达Lyve-1在E10.5，这两种标志物继续在HSEC中表达。尽管已知HSEC和淋巴EC（LECs）具有共同的功能和表型特征，但我们清楚地表明，HSEC可以通过分子标记物的表达和更高的内吞活性与LECs区分开来。我们的研究结果为组织特异性EC的发展和区分HSEC与其他类型EC（包括LEC）的表型标准提供了新的见解。
Endothelial cells (ECs) display distinct structural and functional characteristics depending on the tissue and developmental stage; however, the development of tissue-specific ECs remains poorly understood. Here, we describe the development of hepatic sinusoids in mice based on the expression of hyaluronan receptors Stab2 and Lyve-1. Flk- 1 (+) cells in and around the liver bud begin to express Stab2 at embryonic day (E) 9.5, before the formation of vascular lumen. Hepatic sinusoidal endothelial cells (HSECs) begin to express Lyve-1 at E10.5, and both markers continue to be expressed in HSECs thereafter. Although HSECs and lymphatic ECs (LECs) are known to share functional and phenotypic characteristics, we clearly show that HSECs can be distinguished from LECs by the expression of molecular markers and higher endocytotic activity. Our results provide new insight into the development of tissue-specific ECs and phenotypic criteria to distinguish HSECs from other types of ECs, including LECs.