Stat3 activation in prostatic carcinomas
Stat3 activation in prostatic carcinomas
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DOI：
10.1002/pros.10079
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发表时间：
2002-06-01
期刊：
影响因子：
2.8
通讯作者：
Gao, AC
中科院分区：
文献类型：
--
作者：
Dhir, R;Ni, ZY;Gao, AC
背景活化的Stat 3存在于各种类型的永生细胞系和癌症中。我们和其他人先前已经证明，Stat 3在大鼠和人前列腺癌细胞系中被组成性激活，并且Stat 3激活参与前列腺癌细胞中IL-6介导的信号转导。本研究的目的是检测良性和恶性人类前列腺组织中Stat 3的定量活性，并分析Stat 3活性水平与临床和病理参数之间的相关性。方法。共104人原发性前列腺组织中的Stat 3活性水平进行了分析，使用电迁移率变化测定和磷酸化Stat 3的免疫组织化学染色。所用的组织样本为42例前列腺癌、42例来自前列腺癌患者的匹配的正常前列腺组织（肿瘤旁正常）和20例来自器官供体的正常前列腺组织。与来自无前列腺癌的供体的正常前列腺相比，在前列腺癌和与肿瘤相邻的匹配的正常前列腺组织中检测到显著更高水平的组成性Stat 3活性。肿瘤组织和癌旁正常前列腺组织中Stat 3活性差异无统计学意义。前列腺癌样本中Stat 3活性与Gleason分级或血清PSA水平之间没有相关性。结论。这些结果表明Stat 3在前列腺癌中被组成性激活。在前列腺癌和肿瘤附近的正常前列腺组织中的高水平的Stat 3活性表明，Stat 3活化可能发生在前列腺的可检测的组织学改变之前。
Background. Activated Stat3 is found in various types of immortal cell lines and cancers. We and others have previously demonstrated that Stat3 is constitutively activated in rat and human prostate cancer cell lines, and that Stat3 activation is involved in IL-6-mediated signaling transduction in prostate cancer cells. The aim of this study,is to examine quantitative Stat3 activity in benign and malignant human prostate tissues and analyze the association between Stat3 activity levels and the clinical and pathologic parameters.Methods. Stat3 activity levels were analyzed in a total of 104 human primary prostate tissues using electromobility shift assay and immunohistochemical staining for phosphorylated Stat3. The tissue samples used were 42 prostate carcinomas, 42 matched normal prostate tissues from patients with prostatic adenocarcinoma (normal adjacent to tumor), and 20 normal prostate tissues from organ donors.Results. Significantly higher levels of constitutive Stat3 activity were detected in both prostate carcinomas and the matched normal prostate tissues adjacent to tumors compared to the normal prostates from donors without prostate cancer. There was no significant difference of Stat3 activity in foci of tumor and normal prostate tissue adjacent to tumor. No correlation was seen between Stat3 activity and Gleason grade or serum PSA levels in samples from prostate carcinomas.Conclusions. These results indicate that Stat3 is constitutively activated in prostate cancer. The high level of Stat3 activity in both the prostate carcinomas and the normal prostate tissues adjacent to tumors suggests that Stat3 activation may occur before detectable histological alterations of the prostate.