MIGRATION OF BACTERIA IN SEMISOLID AGAR
MIGRATION OF BACTERIA IN SEMISOLID AGAR
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DOI：
10.1073/pnas.86.18.6973
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发表时间：
1989-09-01
影响因子：
11.1
通讯作者：
BERG, HC
中科院分区：
文献类型：
--
作者：
WOLFE, AJ;BERG, HC
本文研究了大肠杆菌趋化细胞和非趋化细胞在半固体琼脂中的迁移。虽然成群的非趋化性细胞通常小于趋化性细胞，但它们在大小和结构上有显著差异。没有翻滚或不断翻滚的细胞形成了最小的群体。以中间频率翻滚的细胞形成了大得多的群体，即使删除了许多已知的趋化性所需的基因。令人惊讶的是，翻滚频率越高，蜂群越大。显微镜检查显示，翻滚使细胞能够远离琼脂中的障碍物。因此，并非所有有效聚集的细胞都需要趋化性。
We studied the migration through semisolid agar of chemotactic and nonchemotactic cells of Escherichia coli. While swarms of nonchemotactic cells were generally smaller than those of chemotactic cells, they varied markedly in size and in structure. Cells that failed to tumble or that tumbled incessantly formed the smallest swarms. Cells that tumbled at intermediate frequencies formed much large swarms, even when deleted for many of the genes known to be required for chemotaxis. Surprisingly, the higher the tumble frequency, the larger the swarms. Microscopic examination revealed that tumbles enable cells to back away from obstructions in the agar. Thus, not all cells that swarm effectively need be chemotactic.