Analysis of in vitro and in vivo products of the TMV 30kDa open reading frame using antisera raised against a synthetic peptide.
Analysis of in vitro and in vivo products of the TMV 30kDa open reading frame using antisera raised against a synthetic peptide.
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使用针对合成肽产生的抗血清分析 TMV 30kDa 开放阅读框的体外和体内产物。
DOI：
10.1016/0014-5793(83)80316-0
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发表时间：
1983
期刊：
影响因子：
3.5
通讯作者：
Zimmern,David
中科院分区：
文献类型：
--
作者：
Kiberstis,PaulaA;Pessi,Antonello;Atherton,Eric;Jackson,Richard;Hunter,Tony;Zimmern,David
采用固相法合成了TMV 30kDa开放阅读框中预测的c -末端11个氨基酸加上另外一个n -末端Tyr残基的tyrr - ser - glu - ala - thr - val - ala - glu - ser - asp - ser - phe肽，并用于兔抗血清培养。这些抗血清从TMV短棒RNA的体外翻译产物中析出4种主要蛋白（p30, p28， p19和18.5kDa），但只有一种蛋白（明显的tmr = 30500）来自TMV感染的烟草原生质体。该蛋白在感染后8至16小时合成，具有与体外合成的p30相同的[35S] met标记的色氨酸。
The peptide Tyr-Ser-Glu-Ala-Thr-Val-Ala-Glu-Ser-ASP-Ser-Phe (the predicted C-terminal 11 amino acids of the TMV 30kDa open reading frame plus an additional N-terminal Tyr residue) was synthesized by solid phase methods and used to raise antisera in rabbits. These antisera precipitated 4 major proteins (p30, p28, p19 and 18.5kDa) from in vitro translation products of TMV short rod RNA , but only one, of apparentMr= 30500, FROM TMV-infected tobacco protoplasts. This protein was made between 8 and 16 h post infection, and had [35S]Met-labelled tryptic peptides identical to those of in vitro synthesized p30.