Caffeine delays oocyte aging and maintains the quality of aged oocytes safely in mouse.
Caffeine delays oocyte aging and maintains the quality of aged oocytes safely in mouse.
复制标题
咖啡因可以延缓小鼠卵母细胞的衰老并安全地维持老化卵母细胞的质量。
DOI：
10.18632/oncotarget.15292
复制
发表时间：
2017-03-28
期刊：
影响因子：
--
通讯作者：
Miao YL
中科院分区：
文献类型：
--
作者：
Zhang X;Liu X;Chen L;Wu DY;Nie ZW;Gao YY;Miao YL
咖啡因作为一种卵母细胞老化抑制剂，在许多不同的物种中被用来控制或延缓卵母细胞的老化。然而，咖啡因的安全性和咖啡因抑制老化卵母细胞的发育能力还没有得到系统的研究。因此，我们对咖啡因处理的卵母细胞的纺锤体形态、皮质颗粒分布、透明带硬化和原核形成进行了检测，以评估卵母细胞的质量。我们发现，咖啡因处理的老化卵母细胞对激活刺激的敏感性较弱，并在进一步老化6小时后恢复正常。咖啡因维持了卵母细胞纺锤体形态，改变了老化卵母细胞皮质颗粒的分布，但不能阻止卵透明带硬化。此外，咖啡因促进了老化卵母细胞的原核形成，减少了受精后的碎裂。这些结果表明，咖啡因可以安全地维持小鼠老化卵母细胞的质量。
Caffeine, as an oocyte aging inhibitor, was used in many different species to control or delay oocyte aging. However, the safety of caffeine and developmental competence of aged oocytes inhibited by caffeine has not been studied systematically. So we detected the spindle morphology, distribution of cortical granules, zona pellucida hardening and pronucleus formation to assess oocyte quality of caffeine treated oocytes. We found that aged oocytes treated by caffeine maintained weak susceptibility to activating stimuli and regained normal competent after aged further 6 hr. Caffeine maintained the spindle morphology, changed cortical granules distribution of aged oocytes and could not prevent zona pellucida hardening. Furthermore, caffeine increased pronucleus formation of aged oocytes and decreased fragmentation after fertilization. These results suggested that caffeine could maintain the quality of aged oocytes safely in mouse.