Comparative Analysis of Drought-Responsive Transcriptome in Different Genotype Saccharum spontaneum L.
Comparative Analysis of Drought-Responsive Transcriptome in Different Genotype Saccharum spontaneum L.
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DOI：
10.1007/s12355-019-00774-1
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发表时间：
2020-01
期刊：
影响因子：
1.9
通讯作者：
Tian-Ju Wang;Xianhong Wang;Qing Yang
中科院分区：
文献类型：
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作者：
Tian-Ju Wang;Xianhong Wang;Qing Yang
蔗糖spontaneumL。是甘蔗育种材料中最耐旱的植物之一。为阐明干旱胁迫条件下水稻抗旱克隆13-13和干旱敏感克隆84-261的内部分子机制。为提高抗旱性基因inS的研究和利用水平，鉴定了与抗旱性密切相关的天然功能基因。spontaneum。在干旱胁迫7 d后，对该树种的13-13和84-261克隆以及对照叶片进行了高通量转录组测序分析。这两个样本中差异表达非常显著的基因主要参与与非生物胁迫或逆境相关的代谢活动，包括植物激素信号转导、糖酵解/糖异生、淀粉和蔗糖代谢、光合作用和氧化磷酸化。本研究确定了一些抗旱性的关键基因，如参与渗透调节、ROS去除系统、毒素降解酶、次生代谢、信号转导、转录因子以及生物和非生物胁迫的基因。我们推测这些基因可能在抵抗干旱inS中发挥了重要作用。spontaneum。本研究鉴定了两个干旱胁迫样品和对照中基因表达的变化，并获得了DEGs的功能信息。结果表明，13-13的deg值高于84-261。此外，13-13的下调基因多于上调基因，84-261的上调基因多于下调基因。这些结果证实了抗旱性。自发性植物使用某些基因的组成表达来应对干旱胁迫，而干旱敏感。自发性植物仅在干旱胁迫下表达抗旱基因。因此,酒店。自发性无性系13-13的抗旱性强于无性系84-261。研究结果表明，s。自发性干旱胁迫涉及多个基因和多种生物代谢过程的协调调控，表明基因表达的变化可能是该物种应对干旱胁迫的主要调控方式。
Saccharum spontaneumL. is one of the most drought-resistant plants among sugarcane breeding materials. To elucidate the internal molecular mechanisms that occur under drought stress conditions in drought-resistant clone 13-13 and drought-sensitive clone 84-261 ofS. spontaneum, functional genes closely associated with drought resistance were identified to improve the study and utilization of stress resistance genes inS. spontaneum. High-throughput transcriptome sequencing analyses were performed on the 13-13 and 84-261 clones of this species as well as on control leaves of these materials after 7 days of drought stress. The genes in these two samples that displayed very significant differential expression mainly participated in metabolic activities associated with abiotic stress or adversity, including plant hormone signal transduction, glycolysis/gluconeogenesis, starch and sucrose metabolism, photosynthesis, and oxidative phosphorylation. This study identified some key DEGs for drought resistance, such as DEGs involved in the osmotic regulator, ROS removal system, toxin-degrading enzymes, secondary metabolism, signaling, transcription factors, and biotic and abiotic stresses. We speculated that these genes may have played an important role to resist drought inS. spontaneum. This study identified changes in gene expression and obtained functional information on DEGs in two drought-stressed samples and control. The results showed that the 13-13 had more DEGs than the 84-261. In addition, 13-13 had more down-regulated genes than up-regulated genes, whereas 84-261 had more up-regulated genes than down-regulated genes. These results confirm that drought-resistantS. spontaneumuses the constitutive expression of certain genes to respond to drought stress, whereas drought-sensitiveS. spontaneumexpresses drought-resistant genes only during drought stress. Thus, theS. spontaneumclone 13-13 exhibited stronger drought resistance than clone 84-261. The results of this study indicate that the response ofS. spontaneumto drought stress involves the coordinated regulation of multiple genes and multiple biological metabolic processes and suggest that changes in gene expression might be the major regulatory method through which this species copes with drought stress.